PUBLICATION
Auriculasin from Flemingia philippinensis roots shows good therapeutic indexes on hyperactive behavior in zebrafish
- Authors
- Wang, T., Liu, Y., Liu, H., Li, C., Wang, Y.
- ID
- ZDB-PUB-180722-2
- Date
- 2018
- Source
- Biochemical and Biophysical Research Communications 503(3): 1254-1259 (Journal)
- Registered Authors
- Liu, Chunyan, Liu, Huazhen, Wang, Tuoyi
- Keywords
- ADHD, Circadian clock, Hyperactive behavior, Isoflavones, Zebrafish, per1b
- MeSH Terms
-
- Animals
- Attention Deficit Disorder with Hyperactivity/drug therapy*
- Attention Deficit Disorder with Hyperactivity/metabolism
- Fabaceae/chemistry*
- Isoflavones/chemistry
- Isoflavones/isolation & purification
- Isoflavones/pharmacology*
- Locomotion/drug effects*
- Period Circadian Proteins/deficiency
- Period Circadian Proteins/genetics
- Period Circadian Proteins/metabolism
- Plant Roots/chemistry*
- Zebrafish
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 30025896 Full text @ Biochem. Biophys. Res. Commun.
Citation
Wang, T., Liu, Y., Liu, H., Li, C., Wang, Y. (2018) Auriculasin from Flemingia philippinensis roots shows good therapeutic indexes on hyperactive behavior in zebrafish. Biochemical and Biophysical Research Communications. 503(3):1254-1259.
Abstract
Previously, period1b-/- zebrafish mutants were used to establish an attention deficit hyperactivity disorder (ADHD) model, in which hyperactive behavior was found to be a typical characteristic of ADHD due to down-regulated dopamine levels. Here, we used five prenylated isoflavones from Flemingia philippinensis roots to study their therapeutic effects on hyperactivity behavior in period1b-/- zebrafish. Results of locomotor activity assay showed that auriculasin, one of the prenylated isoflavones, significantly reduced the hyperactivity behavior in period1b-/- zebrafish. Hormone measurement results showed that auriculasin increased melatonin and dopamine content. Results of quantitative real-time polymerase chain reaction showed that auriculasin down-regulated the expression of mao but up-regulated the expression of th and per1b. Thus, auriculasin demonstrated a potential biological effect on dopamine activity to inhibit hyperactivity behavior in the ADHD zebrafish model by regulating circadian clock gene per1b.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping