PUBLICATION
A variable undecad repeat domain in cavin1 regulates caveola formation and stability
- Authors
- Tillu, V.A., Lim, Y.W., Kovtun, O., Mureev, S., Ferguson, C., Bastiani, M., McMahon, K.A., Lo, H.P., Hall, T.E., Alexandrov, K., Collins, B.M., Parton, R.G.
- ID
- ZDB-PUB-180720-4
- Date
- 2018
- Source
- EMBO reports 19(9): (Journal)
- Registered Authors
- Hall, Thomas, Lo, Harriet, Parton, Robert G.
- Keywords
- caveolae, cavin, coiledâcoil, undecad repeat
- MeSH Terms
-
- Animals
- Carrier Proteins/metabolism
- Caveolae/metabolism*
- Cell Membrane/metabolism
- DNA Mutational Analysis
- Humans
- Intracellular Signaling Peptides and Proteins/metabolism
- MCF-7 Cells
- Membrane Proteins/chemistry
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Notochord/metabolism
- PC-3 Cells
- RNA-Binding Proteins/chemistry
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism*
- Stress, Mechanical
- Zebrafish
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30021837 Full text @ EMBO Rep.
Citation
Tillu, V.A., Lim, Y.W., Kovtun, O., Mureev, S., Ferguson, C., Bastiani, M., McMahon, K.A., Lo, H.P., Hall, T.E., Alexandrov, K., Collins, B.M., Parton, R.G. (2018) A variable undecad repeat domain in cavin1 regulates caveola formation and stability. EMBO reports. 19(9):.
Abstract
Caveolae are plasma membrane invaginations involved in transport, signalling and mechanical membrane sensing in metazoans. Their formation depends upon multiple interactions between membrane-embedded caveolins, lipids and cytosolic cavin proteins. Of the four cavin family members, only cavin1 is strictly required for caveola formation. Here, we demonstrate that an eleven residue (undecad) repeat sequence (UC1) exclusive to cavin1 is essential for caveolar localization and promotes membrane remodelling through binding to phosphatidylserine. In the notochord of mechanically stimulated zebrafish embryos, the UC1 domain is required for caveolar stability and resistance to membrane stress. The number of undecad repeats in the cavin1 UC1 domain varies throughout evolution, and we find that an increased number also correlates with increased caveolar stability. Lastly, we show that the cavin1 UC1 domain induces dramatic remodelling of the plasma membrane when grafted into cavin2 suggesting an important role in membrane sculpting. Overall, our work defines a novel conserved cavin1 modular domain that controls caveolar assembly and stability.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping