A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish
- Ma, X., Ding, Y., Wang, Y., Xu, X.
- Journal of visualized experiments : JoVE (136): (Journal)
- Registered Authors
- Ding, Yonghe, Ma, Xiao, Xu, Xiaolei
- MeSH Terms
- Cardiomyopathies/chemically induced*
- Disease Models, Animal
- Doxorubicin/adverse effects*
- 29939187 Full text @ J. Vis. Exp.
Ma, X., Ding, Y., Wang, Y., Xu, X. (2018) A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish. Journal of visualized experiments : JoVE. (136):.
The genetically accessible adult zebrafish (Danio rerio) has been increasingly used as a vertebrate model for understanding human diseases such as cardiomyopathy. Because of its convenience and amenability to high throughput genetic manipulations, the generation of acquired cardiomyopathy models, such as the doxorubicin-induced cardiomyopathy (DIC) model in adult zebrafish, is opening the doors to new research avenues, including discovering cardiomyopathy modifiers via forward genetic screening. Different from the embryonic zebrafish DIC model, both initial acute and later chronic phases of cardiomyopathy can be determined in the adult zebrafish DIC model, enabling the study of stage-dependent signaling mechanisms and therapeutic strategies. However, variable results can be obtained with the current model, even in the hands of experienced investigators. To facilitate future implementation of the DIC model, we present a detailed protocol on how to generate this DIC model in adult zebrafish and describe two alternative ways of intraperitoneal (IP) injection. We further discuss options on how to reduce variations to obtain reliable results and provide suggestions on how to appropriately interpret the results.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes