PUBLICATION

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Authors
Muto, A., Kawakami, K.
ID
ZDB-PUB-180622-7
Date
2018
Source
Journal of visualized experiments : JoVE   (136): (Journal)
Registered Authors
Kawakami, Koichi, Muto, Akira
Keywords
none
MeSH Terms
  • Animals
  • Behavior, Animal/physiology*
  • Calcium/chemistry*
  • Larva/physiology*
  • Neurons/physiology*
  • Zebrafish
PubMed
29912192 Full text @ J. Vis. Exp.
Abstract
To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences of blocking its activity in living animals. Laser ablation of neurons is an effective method for this purpose when neurons are selectively labeled with fluorescent probes. In the present study, protocols for laser ablating a subpopulation of neurons using a two-photon microscope and testing of its functional and behavioral consequences are described. In this study, prey capture behavior in zebrafish larvae is used as a study model. The pretecto-hypothalamic circuit is known to underlie this visually-driven prey catching behavior. Zebrafish pretectum were laser-ablated, and neuronal activity in the inferior lobe of the hypothalamus (ILH; the target of the pretectal projection) was examined. Prey capture behavior after pretectal ablation was also tested.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes