PUBLICATION

3D in vivo imaging with extended-focus optical coherence microscopy

Authors
Chen, Y., Trinh, L.A., Fingler, J., Fraser, S.E.
ID
ZDB-PUB-180615-20
Date
2017
Source
Journal of biophotonics   10: 1411-1420 (Journal)
Registered Authors
Fraser, Scott E., Trinh, Le
Keywords
biological imaging, extended focus, microscopy, optical coherence microscopy
MeSH Terms
  • Animals
  • Equipment Design
  • Imaging, Three-Dimensional/methods*
  • Microscopy/instrumentation
  • Microscopy/methods*
  • Quality Control
  • Zebrafish
PubMed
28417564 Full text @ J. Biophotonics
Abstract
Optical coherence microscopy (OCM) has unique advantages of non-invasive 3D imaging without the need of exogenous labels for studying biological samples. However, the imaging depth of this technique is limited by the tradeoff between the depth of focus (DOF) and high lateral resolution in Gaussian optics. To overcome this limitation, we have developed an extended-focus OCM (xf-OCM) imaging system using quasi-Bessel beam illumination to extend the DOF to ∼100 μm, about 3-fold greater than standard OCM. High lateral resolution of 1.6 μm ensured detailed identification of structures within live animal samples. The insensitivity to spherical aberrations strengthened the capability of our xf-OCM system in 3D biological imaging.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes