PUBLICATION
Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer
- Authors
- Bond, S.T., McEwen, K.A., Yoganantharajah, P., Gibert, Y.
- ID
- ZDB-PUB-180614-10
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1797: 393-401 (Chapter)
- Registered Authors
- Gibert, Yann
- Keywords
- Development, Embryo, Metabolism, Mitochondria, Respiration, Seahorse, Zebrafish
- MeSH Terms
-
- Animals
- Cell Respiration
- Embryo, Nonmammalian/metabolism*
- Metabolome*
- Metabolomics*/instrumentation
- Metabolomics*/methods
- Mitochondria/metabolism
- Oxygen Consumption
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 29896705 Full text @ Meth. Mol. Biol.
Citation
Bond, S.T., McEwen, K.A., Yoganantharajah, P., Gibert, Y. (2018) Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer. Methods in molecular biology (Clifton, N.J.). 1797:393-401.
Abstract
The Agilent Seahorse Extracellular Flux Analyzer can be used to measure the oxygen consumption rate (OCR) and extra cellular acidification rate (ECAR), from which mitochondrial bioenergetics measurements can be determined including basal respiration, respiration due to ATP turnover, uncoupled respiration/proton leak, and maximum respiration. This novel method demonstrates how to use a Seahorse XF 24 Extracellular Flux Analyzer to measure the bioenergetic flux of zebrafish embryos in vivo during development. This provides a tool that enables characterization of metabolic parameters in a living organism, utilizing Agilent Islet Capture Microplates where respiration parameters can be compared between controls and genetically altered/pharmacologically treated embryos in real time. This method can be used to analyze and identify novel pharmaceuticals and genes that influence respiration, mitochondrial function, and metabolism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping