PUBLICATION

Selectivity and sensitivity of molybdenum oxide-polycaprolactone nanofiber composites on skin cancer: Preliminary in-vitro and in-vivo implications

Authors
Janani, I., Lakra, R., Kiran, M.S., Korrapati, P.S.
ID
ZDB-PUB-180614-1
Date
2018
Source
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)   49: 60-71 (Journal)
Registered Authors
Keywords
Apoptosis, Electrospinning, Molybdenum oxide, Nanofibers, Skin cancer, Zebra fish model
MeSH Terms
  • Animals
  • Apoptosis/drug effects
  • Calorimetry, Differential Scanning
  • Cell Line
  • Cell Line, Tumor
  • DNA Fragmentation
  • Flow Cytometry
  • Humans
  • Microscopy, Electron, Scanning
  • Molybdenum/chemistry*
  • Nanofibers/chemistry*
  • Nanofibers/therapeutic use
  • Nanoparticles/chemistry*
  • Oxides/chemistry*
  • Polyesters/chemistry*
  • Reactive Oxygen Species/metabolism
  • Real-Time Polymerase Chain Reaction
  • Skin Neoplasms/drug therapy*
  • Skin Neoplasms/metabolism
  • Tissue Engineering
  • Zebrafish
PubMed
29895373 Full text @ J. Trace Elem. Med. Biol.
Abstract
Cancer nanomedicine has emerged as a revolution in the last decade opening up promising strides for the cancer treatment. The major challenge in these therapeutic approaches resides in the failure of clinical trials owing to the immunological cancer microenvironment. Therefore, the success of next generation nanomedicine depends on tunable physicochemical nanomaterial design and corresponding clinical trials by integrating targeted delivery with mitigated toxicity. The present study deals with the fabrication of nanofibrous scaffold impregnated with molybdenum nanoparticles for targeted skin cancer therapeutics. Molybdenum oxide, a transitional metal oxide is gaining rapid importance due to its vital role in cellular and molecular metabolism. Polycaprolactone nanofibers were chosen as a matrix to localize the nanoparticles topically facilitating selective apoptosis of the tumor cells over the normal cells with mitigated side effects. The scaffold was designed to tailor the physicochemical, mechanical and biological suitability for skin cancer (melanoma and non melanoma). The designed scaffold was found to reduce more than 50% cell viability of the cancer cells selectively through apoptosis as confirmed using AO/PI staining and the probable mechanism could be attributed to the induction of mitochondria dependent apoptosis as observed by JC1 dye staining. In-vivo trials in zebra fish were found to reduce cancer progression by more than 30% in 14 days. The fabricated molybdenum trioxide nano constructs not only serve as tunable targeted systems but also open venues capable of ferrying chemotherapeutic drugs sparing normal cells alleviating the trauma due to side effects.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping