PUBLICATION
Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation.
- Authors
- Lee, H., Li, H., Kweon, M., Choi, Y., Kim, M.J., Ryu, J.H.
- ID
- ZDB-PUB-180609-24
- Date
- 2018
- Source
- International Journal of Molecular Sciences 19(6): (Journal)
- Registered Authors
- Keywords
- 3T3-L1 adipocyte, Angelica keiskei, adipogenesis, autophagy, clonal expansion, isobavachalcone
- MeSH Terms
-
- 3T3-L1 Cells
- Adipocytes/cytology
- Adipocytes/drug effects
- Adipocytes/metabolism
- Adipogenesis/drug effects*
- Angelica/chemistry
- Animals
- Anti-Obesity Agents/chemistry
- Anti-Obesity Agents/pharmacology*
- Cell Cycle/drug effects
- Chalcones/chemistry
- Chalcones/pharmacology*
- Lipid Metabolism/drug effects*
- Mice
- Zebrafish
- PubMed
- 29882838 Full text @ Int. J. Mol. Sci.
Citation
Lee, H., Li, H., Kweon, M., Choi, Y., Kim, M.J., Ryu, J.H. (2018) Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation.. International Journal of Molecular Sciences. 19(6).
Abstract
We isolated isobavachalcone (IBC) from Angelica keiskei (AK) as an anti-obesity component. IBC dose-dependently inhibited 3T3-L1 adipocyte differentiation by down-regulating adipogenic factors. At the mitotic clonal expansion stage (MCE), IBC caused cell cycle arrest in G0/G1 with decreased expression of cell cycle-regulating proteins. IBC also inhibited autophagic flux by inducing intracellular accumulation of LC3B and SQSTM1/p62 proteins while decreasing expression levels of regulating factors for autophagy initiation. In parallel with the inhibition of adipocyte differentiation, IBC decreased intrahepatic fat deposits and rescued the liver steatosis in high fat cholesterol diet-fed zebrafish. In this study, we found that IBC isolated from AK suppresses mitotic clonal expansion and autophagy flux of adipocytes and also shows anti-obesity activity in a high cholesterol-diet zebrafish model by decreasing intrahepatic fat deposits. These results suggest that IBC could be a leading pharmacological compound for the development of anti-obesity drugs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping