PUBLICATION
Lasting changes induced by mild alcohol exposure during embryonic development in BDNF, NCAM and synaptophysin positive neurons quantified in adult zebrafish
- Authors
- Mahabir, S., Chatterjee, D., Misquitta, K., Chatterjee, D., Gerlai, R.
- ID
- ZDB-PUB-180531-14
- Date
- 2018
- Source
- The European journal of neuroscience 47(12): 1457-1473 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Fetal alcohol spectrum disorder (FASD), brain plasticity, neuronal protein, prenatal, teratogen
- MeSH Terms
-
- Animals
- Brain/cytology
- Brain/drug effects*
- Brain-Derived Neurotrophic Factor*
- Central Nervous System Depressants/adverse effects*
- Disease Models, Animal
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects*
- Ethanol/adverse effects*
- Fetal Alcohol Spectrum Disorders*
- Neural Cell Adhesion Molecules*
- Neuronal Plasticity/drug effects*
- Neurons/cytology
- Neurons/drug effects*
- Synaptophysin*
- Teratogens/pharmacology*
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 29846983 Full text @ Eur. J. Neurosci.
Citation
Mahabir, S., Chatterjee, D., Misquitta, K., Chatterjee, D., Gerlai, R. (2018) Lasting changes induced by mild alcohol exposure during embryonic development in BDNF, NCAM and synaptophysin positive neurons quantified in adult zebrafish. The European journal of neuroscience. 47(12):1457-1473.
Abstract
Fetal Alcohol Spectrum Disorder (FASD) is one of the leading causes of mental health issues worldwide. Analysis of zebrafish exposed to alcohol during embryonic development confirmed that even low concentrations of alcohol for a short period of time may have lasting behavioral consequences at the adult or old age. The mechanism of this alteration has not been studied. Here, we immersed zebrafish embryos into 1% alcohol solution (vol/vol%) at 24 hours post-fertilization (hpf) for 2 hours, and analyze potential changes using immunohistochemistry. We measured the number of BDNF (brain derived neurotrophic factor) and NCAM (neuronal cell adhesion molecule) positive neurons and the intensity of synaptophysin staining in eight brain regions: lateral zone of the dorsal telencephalic area, medial zone of the dorsal telencephalic area, dorsal nucleus of the ventral telencephalic area, ventral nucleus of the ventral telencephalic area, parvocellular preoptic nucleus, ventral habenular nucleus, corpus cerebella and inferior reticular formation. We found embryonic alcohol exposure to significantly reduce the number of BDNF and NCAM positive cells in all brain areas studied as compared to control. We also found alcohol to significantly reduce the intensity of synaptophysin staining in all brain areas except the cerebellum and preoptic area. These neuroanatomical changes correlated with previously demonstrated reduction of social behavior in embryonic alcohol exposed zebrafish, raising the possibility of a causal link. Given the evolutionary conservation across fish and mammals, we emphasize the implication of our current study for human health: even small amount of alcohol consumption may be unsafe during pregnancy. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping