PUBLICATION
Self-Assembled Fluorescent Nanoprobe Based on Forster Resonance Energy Transfer for Carbon Monoxide in Living Cells and Animals via Ligand Exchange
- Authors
- Jia, R., Song, P., Wang, J., Mai, H., Li, S., Cheng, Y., Wu, S.
- ID
- ZDB-PUB-180530-35
- Date
- 2018
- Source
- Analytical chemistry 90(12): 7117-7121 (Journal)
- Registered Authors
- Wang, Jingjing
- Keywords
- none
- MeSH Terms
-
- A549 Cells
- Animals
- Carbon Monoxide/analysis*
- Cell Survival
- Fluorescence Resonance Energy Transfer*
- Fluorescent Dyes/chemical synthesis
- Fluorescent Dyes/chemistry*
- Humans
- Ligands
- Molecular Structure
- Nanoparticles/chemistry*
- Optical Imaging
- Oxadiazoles/chemical synthesis
- Oxadiazoles/chemistry*
- Particle Size
- Zebrafish/embryology
- PubMed
- 29808996 Full text @ Anal. Chem.
Citation
Jia, R., Song, P., Wang, J., Mai, H., Li, S., Cheng, Y., Wu, S. (2018) Self-Assembled Fluorescent Nanoprobe Based on Forster Resonance Energy Transfer for Carbon Monoxide in Living Cells and Animals via Ligand Exchange. Analytical chemistry. 90(12):7117-7121.
Abstract
Carbon monoxide (CO) is recognized as a biologically essential gaseous neurotransmitter that modulates many physiological processes in living subjects. Currently reported fluorescent probes for CO imaging in cells basically utilize palladium related chemistry which requires complicated synthetic work. Herein we provide a new strategy to construct a fluorescent nanoprobe, NanoCO-1, based on the Forster resonance energy transfer (FRET) mechanism by entrapping the existing dirhodium complex as the energy acceptor and the CO recognition part, and a commonly used nitrobenzoxadiazole (NBD) dye as energy donor into a micelle formed by self-assembly. The exchange of ligands in the dirhodium complex by CO in the nanoprobe disrupts the FRET and leads to the turn-on of fluorescence. The merits of NanoCO-1 including good biocompatibility, selectivity, photostability, and low cytotoxity, render this nanoprobe ability to track CO in living cells, zebrafish embryo, and larvae. Our straightforward approach can be extended to establish the CO fluorescent probes based on adsorption of CO on a variety of metal derivatives.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping