PUBLICATION
Protective effects of cichoric acid on H2O2-induced oxidative injury in hepatocytes and larval zebrafish models.
- Authors
- Ma, J., Li, M., Kalavagunta, P.K., Li, J., He, Q., Zhang, Y., Ahmad, O., Yin, H., Wang, T., Shang, J.
- ID
- ZDB-PUB-180530-25
- Date
- 2018
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 104: 679-685 (Journal)
- Registered Authors
- Keywords
- Cichoric acid, Liver injury, Non-alcoholic fatty liver disease, Oxidative stress
- MeSH Terms
-
- Animals
- Antioxidants/pharmacology*
- Apoptosis/drug effects
- Caffeic Acids/pharmacology*
- Cell Line, Tumor
- Hep G2 Cells
- Hepatocytes/drug effects*
- Hepatocytes/metabolism
- Humans
- Hydrogen Peroxide/pharmacology
- Larva/drug effects*
- Larva/metabolism
- Liver/drug effects
- Liver/metabolism
- Models, Animal
- NF-E2-Related Factor 2/metabolism
- Non-alcoholic Fatty Liver Disease/chemically induced
- Non-alcoholic Fatty Liver Disease/drug therapy*
- Non-alcoholic Fatty Liver Disease/metabolism
- Oxidants/metabolism
- Oxidation-Reduction/drug effects*
- Oxidative Stress/drug effects
- Protective Agents/pharmacology*
- Succinates/pharmacology*
- Superoxide Dismutase/metabolism
- Zebrafish
- PubMed
- 29803928 Full text @ Biomed. Pharmacother.
Citation
Ma, J., Li, M., Kalavagunta, P.K., Li, J., He, Q., Zhang, Y., Ahmad, O., Yin, H., Wang, T., Shang, J. (2018) Protective effects of cichoric acid on H2O2-induced oxidative injury in hepatocytes and larval zebrafish models.. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 104:679-685.
Abstract
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease with a broad spectrum of liver injury. Oxidant stress is believed to be the pathogenesis of NAFLD as the "second hit". Hydrogen peroxide is widely used as an oxidant reagent to induce the oxidant injury of cells and larval zebrafish. Recently, cichoric acid is being studied extensively for its obesity attenuating, hepatic steatosis reduction and anti-oxidant effects. In this study, to identify whether CRA could protect the H2O2 induced oxidant injury via anti-oxidant impact by using L02 and HepG2 hepatocytes as in vitro and larval zebrafish as in vivo injury models, and evaluated the protective and anti-oxidant effects of CRA by pretreated it on both in vitro and in vivo models. CRA was found to reduce the production of ROS and MDA, activate the anti-oxidant enzymes SOD and GSH-px, and pathways Keap1-Nrf2 and HO-1. These results demonstrated that CRA might protect the liver injury by its anti-oxidant effect, which could be a potential therapeutic agent of NAFLD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping