PUBLICATION

Potential adverse outcome pathway (AOP) of silver nanoparticles mediated reproductive toxicity in zebrafish

Authors
Ma, Y.B., Lu, C.J., Junaid, M., Jia, P.P., Yang, L., Zhang, J.H., Pei, D.S.
ID
ZDB-PUB-180530-22
Date
2018
Source
Chemosphere   207: 320-328 (Journal)
Registered Authors
Junaid, Muhammad
Keywords
AgNPs, Apoptosis, Mitochondrial-mediated, Reproduction, Zebrafish
MeSH Terms
  • Adverse Outcome Pathways*
  • Animals
  • Apoptosis/drug effects
  • Germ Cells/drug effects
  • Germ Cells/pathology*
  • Metal Nanoparticles/toxicity*
  • Mitochondria/drug effects
  • Mitochondria/metabolism
  • Oxidative Stress/drug effects*
  • Reactive Oxygen Species/metabolism
  • Reproduction/drug effects*
  • Silver/toxicity*
  • Zebrafish/growth & development*
PubMed
29803881 Full text @ Chemosphere
Abstract
Recently, the augmented utilization of silver nanoparticles (AgNPs) resulted in increasingrates of its release to aquatic environment, which potentially caused adverse effects to aquatic organisms. Therefore, this study investigated - reproductive toxicity and associated potential adverse outcome pathway (AOP) in zebrafish after chronic exposure to AgNPs. To serve the purpose, three-month-old adult zebrafish were exposed to different concentrations (0, 10, 33 and 100 μg/L) of AgNPs for five weeks. Exposure to 33 and 100 μg/L of AgNPs significantly decreased the fecundity in female zebrafish, accompanied by increasing apoptotic cells in the ovarian and testicular tissue using TUNEL assay. Increasing tissue burdens of AgNPs and reactive oxygen species (ROS) production were also found in both ovary and testis after five-week exposure to AgNPs. To explore the mechanism of the apoptotic pathway, the transcription levels of various genes (bax, bcl-2, caspase-3, and caspase-9) associated with the mitochondrion-mediated apoptosis pathway were examined in zebrafish after exposure to AgNPs. The results showed that the expression patterns of all the investigated genes were altered to some extent. These findings demonstrated that AgNPs exposure caused oxidative stress, induced germ cells apoptosis via mitochondrial-dependent pathway, and ultimately impaired the reproduction in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping