PUBLICATION
Long-term Effects of Simulated Microgravity and Vibration Exposure on Skeletal Development in Zebrafish
- Authors
- Franz-Odendaal, T.A., Edsall, S.
- ID
- ZDB-PUB-180526-3
- Date
- 2018
- Source
- Stem cells and development 27(18): 1278-1286 (Journal)
- Registered Authors
- Franz-Odendaal, Tamara
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation/radiation effects
- Embryonic Development/radiation effects
- Humans
- Neural Crest/growth & development*
- Neural Crest/radiation effects
- Organogenesis/radiation effects*
- Skull/growth & development*
- Skull/radiation effects
- Vibration/adverse effects
- Weightlessness/adverse effects
- Weightlessness Simulation/adverse effects
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 29790426 Full text @ Stem Cells Dev.
Citation
Franz-Odendaal, T.A., Edsall, S. (2018) Long-term Effects of Simulated Microgravity and Vibration Exposure on Skeletal Development in Zebrafish. Stem cells and development. 27(18):1278-1286.
Abstract
Most studies utilising fish to study the effects of simulated microgravity (SMG) only observe the effects during the first week of development. They also do not take into account the potential impact on development of vibrations caused by the equipment. Here we analyse the effects of both SMG and vibration on development of the skeleton. We analyse three different exposure durations and starting points that coincide with cranial neural crest cell migration. We use a combination of bone staining and morphometrics to analyse the effects. Our data show that both vibration and SMG affect vertebrae number and body size, however not all vertebrae are equally affected by each treatment. We also show that delayed ossification manifests during development, particularly after SMG exposure, and this translates into buckled and bent bones in adults. This study highlights the large impact of even very short exposure periods when they coincide with critical time points of development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping