PUBLICATION
Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry
- Authors
- Juan, T., Géminard, C., Coutelis, J.B., Cerezo, D., Polès, S., Noselli, S., Fürthauer, M.
- ID
- ZDB-PUB-180518-3
- Date
- 2018
- Source
- Nature communications 9: 1942 (Journal)
- Registered Authors
- Fürthauer, Maximilian, Juan, Thomas, Polès, Sophie
- Keywords
- none
- MeSH Terms
-
- Gene Expression Regulation, Developmental
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Myosins/genetics*
- Myosins/metabolism
- Left-Right Determination Factors/genetics
- Left-Right Determination Factors/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Animals
- Mutation
- Cilia/genetics
- Cilia/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Body Patterning/genetics*
- Cell Polarity/genetics
- Animals, Genetically Modified
- PubMed
- 29769531 Full text @ Nat. Commun.
Citation
Juan, T., Géminard, C., Coutelis, J.B., Cerezo, D., Polès, S., Noselli, S., Fürthauer, M. (2018) Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry. Nature communications. 9:1942.
Abstract
The establishment of left-right (LR) asymmetry is fundamental to animal development, but the identification of a unifying mechanism establishing laterality across different phyla has remained elusive. A cilia-driven, directional fluid flow is important for symmetry breaking in numerous vertebrates, including zebrafish. Alternatively, LR asymmetry can be established independently of cilia, notably through the intrinsic chirality of the acto-myosin cytoskeleton. Here, we show that Myosin1D (Myo1D), a previously identified regulator of Drosophila LR asymmetry, is essential for the formation and function of the zebrafish LR organizer (LRO), Kupffer's vesicle (KV). Myo1D controls the orientation of LRO cilia and interacts functionally with the planar cell polarity (PCP) pathway component VanGogh-like2 (Vangl2), to shape a productive LRO flow. Our findings identify Myo1D as an evolutionarily conserved regulator of animal LR asymmetry, and show that functional interactions between Myo1D and PCP are central to the establishment of animal LR asymmetry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping