PUBLICATION
Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume
- Authors
- Grassini, D.R., Lagendijk, A.K., De Angelis, J.E., Da Silva, J., Jeanes, A., Zettler, N., Bower, N.I., Hogan, B.M., Smith, K.A.
- ID
- ZDB-PUB-180513-7
- Date
- 2018
- Source
- Development (Cambridge, England) 145(12): (Journal)
- Registered Authors
- Da Silva, Jason, De Angelis, Jessica, Grassini, Daniela, Hogan, Ben M., Jeanes, Angela, Smith, Kelly
- Keywords
- ANF, Cardiac development, Cardiac jelly, Nppa, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Atrial Natriuretic Factor/genetics*
- Bone Morphogenetic Protein 4/genetics
- Bone Morphogenetic Protein 4/metabolism
- Gene Editing
- Heart/embryology*
- Heart Defects, Congenital/genetics
- Hyaluronan Synthases/metabolism
- Natriuretic Peptide, Brain/genetics*
- Receptors, Atrial Natriuretic Factor/genetics*
- T-Box Domain Proteins/metabolism
- Versicans/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 29752386 Full text @ Development
Citation
Grassini, D.R., Lagendijk, A.K., De Angelis, J.E., Da Silva, J., Jeanes, A., Zettler, N., Bower, N.I., Hogan, B.M., Smith, K.A. (2018) Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume. Development (Cambridge, England). 145(12).
Abstract
Atrial natriuretic peptide (nppa/anf) and brain natriuretic peptide (nppb/bnp) form a gene cluster with expression in the chambers of the developing heart. Despite restricted expression, a function in cardiac development has not been demonstrated by mutant analysis. This is attributed to functional redundancy; however, their genomic location in cis has impeded formal analysis. Using genome editing, we have generated mutants for nppa and nppb, and found that single mutants were indistinguishable from wild type, whereas nppa/nppb double mutants displayed heart morphogenesis defects and pericardial oedema. Analysis of atrioventricular canal (AVC) markers show expansion of bmp4, tbx2b, has2 and versican expression into the atrium of double mutants. This expanded expression correlates with increased extracellular matrix in the atrium. Using a biosensor for hyaluronic acid to measure the cardiac jelly (cardiac extracellular matrix), we confirmed cardiac jelly expansion in nppa/nppb double mutants. Finally, bmp4 knockdown rescued the expansion of has2 expression and cardiac jelly in double mutants. This definitively shows that nppa and nppb function redundantly during cardiac development to restrict gene expression to the AVC, preventing excessive cardiac jelly synthesis in the atrial chamber.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping