PUBLICATION
Zebrafish as a model to study bone maturation: Nanoscale structural and mechanical characterization of age-related changes in the zebrafish vertebral column
- Authors
- Chang, Z., Chen, P.Y., Chuang, Y.J., Akhtar, R.
- ID
- ZDB-PUB-180511-11
- Date
- 2018
- Source
- Journal of the Mechanical Behavior of Biomedical Materials 84: 54-63 (Journal)
- Registered Authors
- Chuang, Yung-Jen
- Keywords
- AFM, Age, Bone, EDX, Mechanical properties, Nanomechanics, PF-QNM, Vertebral column, Zebrafish
- MeSH Terms
-
- Aging/physiology*
- Animals
- Biomechanical Phenomena
- Materials Testing
- Mechanical Phenomena*
- Nanotechnology*
- Osteogenesis*
- Spine/physiology*
- Zebrafish*
- PubMed
- 29747057 Full text @ J. Mech. Behav. Biomed. Mater.
Citation
Chang, Z., Chen, P.Y., Chuang, Y.J., Akhtar, R. (2018) Zebrafish as a model to study bone maturation: Nanoscale structural and mechanical characterization of age-related changes in the zebrafish vertebral column. Journal of the Mechanical Behavior of Biomedical Materials. 84:54-63.
Abstract
Zebrafish (Danio rerio) is a useful model for understanding biomedical properties of bone and are widely employed in developmental and genetics studies. Here, we have studied the development of zebrafish vertebral bone at the nanoscale from adolescence (6 months), early adulthood (10 months) to mid-life (14 months). Characterization of the bone was conducted using energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and PeakForce QNM atomic force microscopy (AFM) techniques. SEM and AFM revealed a lamellar structure with mineralized collagen fibrils. There was a significant increase in the wall thickness from 6 to 10 months (76%) and 10 months to 14 months (26%), which is positively correlated with nanomechanical behavior. An increase in the Ca/P ratio was found which was also positively correlated with nanomechanical properties. The change in mechanical properties and Ca/P are similar to those expected in humans when transitioning from adolescence to mid-life. We suggest that zebrafish serve as a suitable model for further studies on age-related changes in bone ultrastructure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping