PUBLICATION
Repeatability and Reproducibility of In Vivo Cone Density Measurements in the Adult Zebrafish Retina
- Authors
- Huckenpahler, A., Wilk, M., Link, B., Carroll, J., Collery, R.
- ID
- ZDB-PUB-180504-6
- Date
- 2018
- Source
- Advances in experimental medicine and biology 1074: 151-156 (Chapter)
- Registered Authors
- Collery, Ross, Link, Brian
- Keywords
- Cone Density, Cone Mosaic, Optical Coherence Tomography (OCT), Photoreceptor, Repeatability, Reproducibility, Zebrafish
- MeSH Terms
-
- Animals
- Artifacts
- Cell Count
- Image Processing, Computer-Assisted/methods
- Reproducibility of Results
- Retina/cytology*
- Retinal Cone Photoreceptor Cells*
- Tomography, Optical Coherence/methods*
- Ultraviolet Rays
- Zebrafish/anatomy & histology*
- PubMed
- 29721939 Full text @ Adv. Exp. Med. Biol.
Citation
Huckenpahler, A., Wilk, M., Link, B., Carroll, J., Collery, R. (2018) Repeatability and Reproducibility of In Vivo Cone Density Measurements in the Adult Zebrafish Retina. Advances in experimental medicine and biology. 1074:151-156.
Abstract
Zebrafish (Danio rerio) are widely used as an experimental model for a wide range of retinal diseases. Previously, optical coherence tomography (OCT) was introduced for quantitative analysis of the zebrafish cone photoreceptor cell mosaic; however no data exists on the intersession reproducibility or intrasession repeatability of such measurements. We imaged 14 wild-type (WT) fish three times each, with 48 h between each time point. En face images of the UV cone mosaic were generated from the OCT volume scans at each time point. These images were then aligned and the overlapping area cropped for analysis. Using a semiautomated cone-counting algorithm, a single observer identified each cone to calculate the cone density for every image, counting each image twice (84 total counts). The OCT cone density measurements were found to have an intersession reproducibility of 0.9988 (95% CI = 0.9978-0.9999) and an intrasession repeatability of 136.0 ± 10.5 cones/mm2 (about 0.7%). Factors affecting image quality include gill movement during acquisition of the OCT volume and variable inclusion of non-UV cone mosaics in the contours used to generate the en face images.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping