PUBLICATION
Image velocimetry and spectral analysis enable quantitative characterization of larval zebrafish gut motility
- Authors
- Ganz, J., Baker, R.P., Hamilton, M.K., Melancon, E., Diba, P., Eisen, J.S., Parthasarathy, R.
- ID
- ZDB-PUB-180504-10
- Date
- 2018
- Source
- Neurogastroenterology and motility 30(9): e13351 (Journal)
- Registered Authors
- Eisen, Judith S., Ganz, Julia
- Keywords
- Hirschsprung disease, enteric nervous system, intestinal motility
- MeSH Terms
-
- Animals
- Enteric Nervous System/physiology
- Gastrointestinal Motility/physiology*
- Image Processing, Computer-Assisted/methods*
- Imaging, Three-Dimensional/methods*
- Larva/physiology*
- Microscopy, Interference/methods*
- Rheology/methods*
- Zebrafish
- PubMed
- 29722095 Full text @ Neurogastroenterol. Motil.
Citation
Ganz, J., Baker, R.P., Hamilton, M.K., Melancon, E., Diba, P., Eisen, J.S., Parthasarathy, R. (2018) Image velocimetry and spectral analysis enable quantitative characterization of larval zebrafish gut motility. Neurogastroenterology and motility. 30(9):e13351.
Abstract
Background Normal gut function requires rhythmic and coordinated movements that are affected by developmental processes, physical and chemical stimuli, and many debilitating diseases. The imaging and characterization of gut motility, especially regarding periodic, propagative contractions driving material transport, are therefore critical goals. Previous image analysis approaches have successfully extracted properties related to the temporal frequency of motility modes, but robust measures of contraction magnitude, especially from in vivo image data, remain challenging to obtain.
Methods We developed a new image analysis method based on image velocimetry and spectral analysis that reveals temporal characteristics such as frequency and wave propagation speed, while also providing quantitative measures of the amplitude of gut motion.
Key results We validate this approach using several challenges to larval zebrafish, imaged with differential interference contrast microscopy. Both acetylcholine exposure and feeding increase frequency and amplitude of motility. Larvae lacking enteric nervous system gut innervation show the same average motility frequency, but reduced and less variable amplitude compared to wild types.
Conclusions & inferences Our image analysis approach enables insights into gut dynamics in a wide variety of developmental and physiological contexts and can also be extended to analyze other types of cell movements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping