|ZFIN ID: ZDB-PUB-180428-5|
Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code
Brown, W., Liu, J., Tsang, M., Deiters, A.
|Source:||Chembiochem : a European journal of chemical biology 19(12): 1244-1249 (Other)|
|Registered Authors:||Tsang, Michael|
|Keywords:||DNA recombination, lineage tracing, optogenetics, unnatural amino acid, zebrafish|
|PubMed:||29701891 Full text @ Chembiochem|
Brown, W., Liu, J., Tsang, M., Deiters, A. (2018) Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code. Chembiochem : a European journal of chemical biology. 19(12):1244-1249.
ABSTRACTCell lineage tracing is used to study embryo development, stem cell differentiation, and to document tumor cell heterogeneity. Cre recombinase-mediated cell labelling is the preferred approach; however, its utility is restricted by when and where DNA recombination takes place. We generated a photoactivatable Cre recombinase by replacing a critical residue in its active site with a photocaged lysine derivative through genetic code expansion in zebrafish embryos. This allows for high spatiotemporal control of DNA recombination using 405 nm light. Importantly, no background activity is seen before irradiation, and after light-triggered removal of the caging group, Cre recombinase activity is restored. We demonstrate the utility of this tool as a cell lineage tracer through activation in different regions and at different time-points in the early embryo. Direct control of Cre recombinase with light will allow for more precise DNA recombination for more nuanced studies of metazoan development and disease.
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