PUBLICATION
Multicoloured fluorescent indicators for live-cell and in vivo imaging of inorganic mercury dynamics
- Authors
- Tao, R., Shi, M., Zou, Y., Cheng, D., Wang, Q., Liu, R., Wang, A., Zhu, J., Deng, L., Hu, H., Chen, X., Du, J., Zhu, W., Zhao, Y., Yang, Y.
- ID
- ZDB-PUB-180424-7
- Date
- 2018
- Source
- Free radical biology & medicine 121: 26-37 (Journal)
- Registered Authors
- Du, Jiu Lin
- Keywords
- in vivo imaging, inorganic mercury, live-cell imaging, multicoloured fluorescent indicators, oxidative damage
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Biosensing Techniques*
- DNA-Binding Proteins/genetics
- Escherichia coli/metabolism
- Fluorescent Dyes/chemistry*
- Fluorescent Dyes/metabolism
- HeLa Cells
- Humans
- Image Processing, Computer-Assisted/methods*
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Mercury/analysis*
- Mercury/toxicity*
- Microscopy, Fluorescence
- Mitochondria/drug effects
- Mitochondria/metabolism
- Mitochondria/pathology*
- Oxidation-Reduction
- Oxygen Consumption
- Zebrafish/metabolism
- PubMed
- 29684410 Full text @ Free Radic. Biol. Med.
Citation
Tao, R., Shi, M., Zou, Y., Cheng, D., Wang, Q., Liu, R., Wang, A., Zhu, J., Deng, L., Hu, H., Chen, X., Du, J., Zhu, W., Zhao, Y., Yang, Y. (2018) Multicoloured fluorescent indicators for live-cell and in vivo imaging of inorganic mercury dynamics. Free radical biology & medicine. 121:26-37.
Abstract
Engineered fluorescent indicators for visualizing mercury ion (Hg2+) are powerful tools to illustrate the intracellular distribution and serious toxicity of the ion. However, the sensitive and specific detection of Hg2+ in living cells and in vivo is challenging. This paper reported the development of fluorescent indicators for Hg2+ in green or red color by inserting a circularly permuted fluorescent protein into a highly mercury-specific repressor. These sensors provided a rapid, sensitive, specific, and real-time read-out of Hg2+ dynamics in solutions, bacteria, subcellular organelles of mammalian cells, and zebrafish, thereby providing a useful new method for Hg2+ detection and bioimaging. In conjunction with the hydrogen peroxide sensor HyPer, we found mercury uptake would trigger subcellular oxidative events at the single-cell level, and provided visual evidence of the causality of mercury and oxidative damage. These sensors would paint the landscape of mercury toxicity to cell functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping