PUBLICATION

Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos

Authors
Chang, H., Yeo, J., Kim, J.G., Kim, H., Lim, J., Lee, M., Kim, H.H., Ohk, J., Jeon, H.Y., Lee, H., Jung, H., Kim, K.W., Kim, V.N.
ID
ZDB-PUB-180407-7
Date
2018
Source
Molecular Cell   70: 72-82.e7 (Journal)
Registered Authors
Jeon, Hee-Yeon, Lee, Hyunsook
Keywords
RNA decay, TAIL-seq, TUT4, TUT7, U tail, Zcchc11, Zcchc6, maternal-to-zygotic transition, poly(A) tail, uridylation
Datasets
GEO:GSE111152
MeSH Terms
  • Embryo, Nonmammalian/enzymology*
  • RNA Processing, Post-Transcriptional
  • Embryo, Mammalian/enzymology*
  • Xenopus laevis/embryology
  • Xenopus laevis/genetics*
  • Xenopus laevis/metabolism
  • Xenopus Proteins/genetics
  • Xenopus Proteins/metabolism
  • Animals
  • RNA Stability*
  • Drosophila melanogaster/embryology
  • Drosophila melanogaster/enzymology
  • Drosophila melanogaster/genetics
  • Nucleotidyltransferases/genetics
  • Nucleotidyltransferases/metabolism*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism*
  • Gastrulation
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Gestational Age
  • Mice, Inbred ICR
  • Transcriptome*
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Animals, Genetically Modified
(all 28)
PubMed
29625039 Full text @ Mol. Cell
Abstract
During the maternal-to-zygotic transition (MZT), maternal RNAs are actively degraded and replaced by newly synthesized zygotic transcripts in a highly coordinated manner. However, it remains largely unknown how maternal mRNA decay is triggered in early vertebrate embryos. Here, through genome-wide profiling of RNA abundance and 3' modification, we show that uridylation is induced at the onset of maternal mRNA clearance. The temporal control of uridylation is conserved in vertebrates. When the homologs of terminal uridylyltransferases TUT4 and TUT7 (TUT4/7) are depleted in zebrafish and Xenopus, maternal mRNA clearance is significantly delayed, leading to developmental defects during gastrulation. Short-tailed mRNAs are selectively uridylated by TUT4/7, with the highly uridylated transcripts degraded faster during the MZT than those with unmodified poly(A) tails. Our study demonstrates that uridylation plays a crucial role in timely mRNA degradation, thereby allowing the progression of early development.
Genes / Markers
Figures
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Expression
No data available
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
tut4MO1-tut4MRPHLNO
tut4MO3-tut4MRPHLNO
tut7MO2-tut7MRPHLNO
tut7MO3-tut7MRPHLNO
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Fish
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Antibodies
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Orthology
Gene Orthology
tut4
tut7
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Engineered Foreign Genes
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Mapping
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