PUBLICATION
Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos
- Authors
- Chang, H., Yeo, J., Kim, J.G., Kim, H., Lim, J., Lee, M., Kim, H.H., Ohk, J., Jeon, H.Y., Lee, H., Jung, H., Kim, K.W., Kim, V.N.
- ID
- ZDB-PUB-180407-7
- Date
- 2018
- Source
- Molecular Cell 70: 72-82.e7 (Journal)
- Registered Authors
- Jeon, Hee-Yeon, Lee, Hyunsook
- Keywords
- RNA decay, TAIL-seq, TUT4, TUT7, U tail, Zcchc11, Zcchc6, maternal-to-zygotic transition, poly(A) tail, uridylation
- Datasets
- GEO:GSE111152
- MeSH Terms
-
- Embryo, Mammalian/enzymology*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Gestational Age
- Xenopus Proteins/genetics
- Xenopus Proteins/metabolism
- Animals, Genetically Modified
- RNA Processing, Post-Transcriptional
- Drosophila melanogaster/embryology
- Drosophila melanogaster/enzymology
- Drosophila melanogaster/genetics
- RNA Stability*
- Xenopus laevis/embryology
- Xenopus laevis/genetics*
- Xenopus laevis/metabolism
- Animals
- Nucleotidyltransferases/genetics
- Nucleotidyltransferases/metabolism*
- Gastrulation
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Mice, Inbred ICR
- Transcriptome*
- Embryo, Nonmammalian/enzymology*
- Gene Expression Regulation, Developmental
- PubMed
- 29625039 Full text @ Mol. Cell
Citation
Chang, H., Yeo, J., Kim, J.G., Kim, H., Lim, J., Lee, M., Kim, H.H., Ohk, J., Jeon, H.Y., Lee, H., Jung, H., Kim, K.W., Kim, V.N. (2018) Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos. Molecular Cell. 70:72-82.e7.
Abstract
During the maternal-to-zygotic transition (MZT), maternal RNAs are actively degraded and replaced by newly synthesized zygotic transcripts in a highly coordinated manner. However, it remains largely unknown how maternal mRNA decay is triggered in early vertebrate embryos. Here, through genome-wide profiling of RNA abundance and 3' modification, we show that uridylation is induced at the onset of maternal mRNA clearance. The temporal control of uridylation is conserved in vertebrates. When the homologs of terminal uridylyltransferases TUT4 and TUT7 (TUT4/7) are depleted in zebrafish and Xenopus, maternal mRNA clearance is significantly delayed, leading to developmental defects during gastrulation. Short-tailed mRNAs are selectively uridylated by TUT4/7, with the highly uridylated transcripts degraded faster during the MZT than those with unmodified poly(A) tails. Our study demonstrates that uridylation plays a crucial role in timely mRNA degradation, thereby allowing the progression of early development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping