PUBLICATION
Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes
- Authors
- Williams, M.L.K., Sawada, A., Budine, T., Yin, C., Gontarz, P., Solnica-Krezel, L.
- ID
- ZDB-PUB-180405-9
- Date
- 2018
- Source
- Nature communications 9: 1319 (Journal)
- Registered Authors
- Budine, Terin, Sawada, Atsushi, Solnica-Krezel, Lilianna, Williams, Margot, Yin, Chunyue
- Keywords
- none
- Datasets
- GEO:GSE96575, GEO:GSE96576
- MeSH Terms
-
- Animals
- Body Patterning
- Cell Adhesion
- Cell Communication
- Chromatin/chemistry
- Crosses, Genetic
- Erythroid-Specific DNA-Binding Factors/genetics*
- Erythroid-Specific DNA-Binding Factors/physiology*
- Gene Expression Regulation, Developmental*
- Membrane Glycoproteins/physiology
- Mutation
- Notochord/physiology
- Sequence Analysis, RNA
- Signal Transduction
- Xenopus
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- PubMed
- 29615614 Full text @ Nat. Commun.
Citation
Williams, M.L.K., Sawada, A., Budine, T., Yin, C., Gontarz, P., Solnica-Krezel, L. (2018) Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes. Nature communications. 9:1319.
Abstract
Anteroposterior (AP) axis extension during gastrulation requires embryonic patterning and morphogenesis to be spatiotemporally coordinated, but the underlying genetic mechanisms remain poorly understood. Here we define a role for the conserved chromatin factor Gon4l, encoded by ugly duckling (udu), in coordinating tissue patterning and axis extension during zebrafish gastrulation through direct positive and negative regulation of gene expression. Although identified as a recessive enhancer of impaired axis extension in planar cell polarity (PCP) mutants, udu functions in a genetically independent, partially overlapping fashion with PCP signaling to regulate mediolateral cell polarity underlying axis extension in part by promoting notochord boundary formation. Gon4l limits expression of the cell-cell and cell-matrix adhesion molecules EpCAM and Integrinα3b, excesses of which perturb the notochord boundary via tension-dependent and -independent mechanisms, respectively. By promoting formation of this AP-aligned boundary and associated cell polarity, Gon4l cooperates with PCP signaling to coordinate morphogenesis along the AP embryonic axis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping