ZFIN ID: ZDB-PUB-180401-1
A novel transgenic zebrafish line for red opsin expression in outer segments of photoreceptor cells
Crespo, C., Soroldoni, D., Knust, E.
Date: 2018
Source: Developmental dynamics : an official publication of the American Association of Anatomists   247(7): 951-959 (Journal)
Registered Authors: Crespo, Cátia, Knust, Elisabeth, Soroldoni, Daniele
Keywords: LWS1, LWS2, cone cells, development, retina
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics*
  • Cone Opsins/genetics
  • Cone Opsins/metabolism
  • Opsins/metabolism*
  • Photoreceptor Cells, Vertebrate/chemistry*
  • Promoter Regions, Genetic
  • Retinal Photoreceptor Cell Outer Segment/chemistry
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 29603474 Full text @ Dev. Dyn.
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ABSTRACT
Opsins are a group of light sensitive proteins present in photoreceptor cells, which convert the energy of photons into electrochemical signals, thus allowing vision. Given their relevance, we aimed to visualise the two red opsins at subcellular scale in photoreceptor cells.
We generated novel zebrafish BAC transgenic lines, which express fluorescently-tagged, full-length Opsin 1 long-wave-sensitive 1 and full-length Opsin 1 long-wave-sensitive 2 under the control of their endogenous promotors. Both fusion proteins are localised in the outer segments of photoreceptor cells. During development, Opn1lw2-mKate2 is detected from the initial formation of outer segments onwards. In contrast, Opn1lw1-mNeonGreen is first detected in juvenile zebrafish at about 2 weeks post fertilisation and both opsins continue to be expressed throughout adulthood. Importantly, the presence of the transgene did not significantly alter the size of outer segments.
We have generated multiple transgenes that mimic the endogenous expression pattern of Opn1lw1 and Opn1lw2 in the developing and adult retina. In contrast to existing lines, our transgene design allows to follow protein localisation. Hence, we expect that these lines could act as useful real-time reporters to directly measure phenomena in retinal development and disease models. This article is protected by copyright. All rights reserved.
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