PUBLICATION
Whole-organism phenotypic screening for anti-infectives promoting host health
- Authors
- Clatworthy, A.E., Romano, K.P., Hung, D.T.
- ID
- ZDB-PUB-180321-25
- Date
- 2018
- Source
- Nature Chemical Biology 14(4): 331-341 (Review)
- Registered Authors
- Clatworthy, Anne, Hung, Deborah
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Infective Agents/pharmacology*
- Bacteria/drug effects
- Bacterial Infections/drug therapy*
- Bacterial Infections/pathology
- Caenorhabditis elegans
- Drosophila melanogaster
- Drug Resistance, Bacterial*
- Humans
- Mice
- Phenotype
- Virulence
- Zebrafish
- PubMed
- 29556098 Full text @ Nat. Chem. Biol.
Citation
Clatworthy, A.E., Romano, K.P., Hung, D.T. (2018) Whole-organism phenotypic screening for anti-infectives promoting host health. Nature Chemical Biology. 14(4):331-341.
Abstract
To date, antibiotics have been identified on the basis of their ability to kill bacteria or inhibit their growth rather than directly for their capacity to improve clinical outcomes of infected patients. Although historically successful, this approach has led to the development of an antibiotic armamentarium that suffers from a number of shortcomings, including the inevitable emergence of resistance and, in certain infections, suboptimal efficacy leading to long treatment durations, infection recurrence, or high mortality and morbidity rates despite apparent bacterial sterilization. Conventional antibiotics fail to address the complexities of in vivo bacterial physiology and virulence, as well as the role of the host underlying the complex, dynamic interactions that cause disease. New interventions are needed, aimed at host outcome rather than microbiological cure. Here we review the role of screening models for cellular and whole-organism infection, including worms, flies, zebrafish, and mice, to identify novel therapeutic strategies and discuss their future implications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping