PUBLICATION
Expression profile of glucose transport-related genes under chronic and acute exposure to growth hormone in zebrafish
- Authors
- Dalmolin, C., Almeida, D.V., Figueiredo, M.A., Marins, L.F.
- ID
- ZDB-PUB-180314-1
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 221: 1-6 (Journal)
- Registered Authors
- Almeida, Daniela Volcan, Figueiredo, Marcio de Azevedo, Marins, Luis Fernando
- Keywords
- Danio rerio, GLUT, Growth hormone, Transgenic
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biological Transport
- Brain/metabolism
- Energy Metabolism
- Gene Expression Profiling*
- Glucose/metabolism*
- Growth Hormone/administration & dosage*
- Growth Hormone/genetics
- Growth Hormone/metabolism*
- Male
- Monosaccharide Transport Proteins/genetics*
- Recombinant Proteins/administration & dosage
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 29530717 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Dalmolin, C., Almeida, D.V., Figueiredo, M.A., Marins, L.F. (2018) Expression profile of glucose transport-related genes under chronic and acute exposure to growth hormone in zebrafish. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 221:1-6.
Abstract
The brain is a highly demanding organ in terms of energy requirements, and precise regulatory mechanisms must operate to ensure adequate energy delivery to maintain normal neuronal activity. Of the energy-promoting substrates present in the circulation, glucose is preferred by the brain, and as with all other substrates, its utilization depends on the presence of humoral factors such as hormones including growth hormone (GH). Glucose enters the cells though specific transport proteins. Among all transporter families and subtypes described to date, the most studied ones are the glucose transporters (GLUTs). The aim of this study is to determine a possible relationship between GH and GLUTs. Therefore, we evaluated the effect of GH-transgenesis and recombinant GH injections upon GLUT expression in the brain of male zebrafish. Overall, the results demonstrated that increasing the GH concentrations above the normal level, via transgenesis or injection, in the fish may impair energy uptake by the brain. This appeared to occur through downregulation of most of the analyzed GLUTs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping