PUBLICATION
LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens.
- Authors
- Yokoyama, C.C., Baldridge, M.T., Leung, D.W., Zhao, G., Desai, C., Liu, T.C., Diaz-Ochoa, V.E., Huynh, J.P., Kimmey, J.M., Sennott, E.L., Hole, C.R., Idol, R.A., Park, S., Storek, K.M., Wang, C., Hwang, S., Milam, A.V., Chen, E., Kerrinnes, T., Starnbach, M.N., Handley, S.A., Mysorekar, I.U., Allen, P.M., Monack, D.M., Dinauer, M.C., Doering, T.L., Tsolis, R.M., Dworkin, J.E., Stallings, C.L., Amarasinghe, G.K., Micchelli, C.A., Virgin, H.W.
- ID
- ZDB-PUB-180303-5
- Date
- 2018
- Source
- The Journal of biological chemistry 293(16): 6022-6038 (Journal)
- Registered Authors
- Chen, Eric
- Keywords
- animal model, immunology, infection, inflammation, membrane protein, mouse, mouse genetics, protein motif
- MeSH Terms
-
- Animals
- Autoantigens/analysis
- Bacterial Infections/genetics
- Bacterial Infections/immunology
- CRISPR-Cas Systems
- Female
- Gene Deletion*
- Immunity, Innate
- Inflammation/genetics
- Inflammation/immunology
- Male
- Membrane Proteins/analysis
- Mice
- Mycoses/genetics
- Mycoses/immunology
- Phylogeny
- Virus Diseases/genetics
- Virus Diseases/immunology
- PubMed
- 29496999 Full text @ J. Biol. Chem.
Citation
Yokoyama, C.C., Baldridge, M.T., Leung, D.W., Zhao, G., Desai, C., Liu, T.C., Diaz-Ochoa, V.E., Huynh, J.P., Kimmey, J.M., Sennott, E.L., Hole, C.R., Idol, R.A., Park, S., Storek, K.M., Wang, C., Hwang, S., Milam, A.V., Chen, E., Kerrinnes, T., Starnbach, M.N., Handley, S.A., Mysorekar, I.U., Allen, P.M., Monack, D.M., Dinauer, M.C., Doering, T.L., Tsolis, R.M., Dworkin, J.E., Stallings, C.L., Amarasinghe, G.K., Micchelli, C.A., Virgin, H.W. (2018) LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens.. The Journal of biological chemistry. 293(16):6022-6038.
Abstract
Germline-encoded receptors recognizing common pathogen-associated molecular patterns are a central element of the innate immune system and play an important role in shaping the host response to infection. Many of the innate immune molecules central to these signaling pathways are evolutionarily conserved. LysMD3 is a novel molecule containing a putative peptidoglycan-binding domain that has orthologs in humans, mice, zebrafish, flies, and worms. We found that the lysin motif (LysM) of LysMD3 is likely related to a previously described peptidoglycan-binding LysM found in bacteria. Mouse LysMD3 is a type II integral membrane protein that co-localizes with GM130+ structures, consistent with localization to the Golgi apparatus. We describe here two lines of mLysMD3-deficient mice for in vivo characterization of mLysMD3 function. We found that mLysMD3-deficient mice were born at Mendelian ratios and had no obvious pathological abnormalities. They also exhibited no obvious immune response deficiencies in a number of models of infection and inflammation. mLysMD3-deficient mice exhibited no signs of intestinal dysbiosis by 16S analysis or alterations in intestinal gene expression by RNA sequencing. We conclude that mLysMD3 contains a LysM with cytoplasmic orientation, but we were unable to define a physiological role for the molecule in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping