ZFIN ID: ZDB-PUB-180228-17
Integrative whole-brain neuroscience in larval zebrafish
Vanwalleghem, G.C., Ahrens, M.B., Scott, E.K.
Date: 2018
Source: Current opinion in neurobiology   50: 136-145 (Review)
Registered Authors: Ahrens, Misha, Scott, Ethan
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain*/diagnostic imaging
  • Brain*/growth & development
  • Brain*/metabolism
  • Calcium/metabolism
  • Larva*
  • Microscopy, Fluorescence
  • Neuroimaging
  • Neurosciences/methods*
  • Zebrafish
PubMed: 29486425 Full text @ Curr. Opin. Neurobiol.
Due to their small size and transparency, zebrafish larvae are amenable to a range of fluorescence microscopy techniques. With the development of sensitive genetically encoded calcium indicators, this has extended to the whole-brain imaging of neural activity with cellular resolution. This technique has been used to study brain-wide population dynamics accompanying sensory processing and sensorimotor transformations, and has spurred the development of innovative closed-loop behavioral paradigms in which stimulus-response relationships can be studied. More recently, microscopes have been developed that allow whole-brain calcium imaging in freely swimming and behaving larvae. In this review, we highlight the technologies underlying whole-brain functional imaging in zebrafish, provide examples of the sensory and motor processes that have been studied with this technique, and discuss the need to merge data from whole-brain functional imaging studies with neurochemical and anatomical information to develop holistic models of functional neural circuits.