Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina
- Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., Corre, R., Bourrat, F., Joly, J.S., Jamen, F.
- Developmental Biology 437(1): 1-16 (Journal)
- Registered Authors
- Bourrat, Franck, Brombin, Alessandro, Jamen, Francoise, Joly, Jean-Stephane, Simion, Matthieu
- Danio rerio, Ribosome biogenesis, cell cycle regulation, differentiation, neural progenitors, optic tectum
- MeSH Terms
- Cell Differentiation/genetics*
- Chromosomal Proteins, Non-Histone/metabolism*
- RNA, Ribosomal/metabolism
- S Phase/genetics*
- 29477341 Full text @ Dev. Biol.
Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., Corre, R., Bourrat, F., Joly, J.S., Jamen, F. (2018) Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina. Developmental Biology. 437(1):1-16.
Fibrillarin (Fbl) is a highly conserved protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. In cellular models, FBL was shown to play an important role in tumorigenesis and stem cell differentiation. We used the zebrafish as an in vivo model to study Fbl function during embryonic development. We show here that the optic tectum and the eye are severely affected by Fbl depletion whereas ventral regions of the brain are less impacted. The morphogenesis defects are associated with impaired neural differentiation and massive apoptosis. Polysome gradient experiments show that fbl mutant larvae display defects in ribosome biogenesis and activity. Strikingly, flow cytometry analyses revealed different S-phase profiles between wild-type and mutant cells, suggesting a defect in S-phase progression.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes