PUBLICATION
Recovery of shape and size in a developing organ pair
- Authors
- Green, A.A., Mosaliganti, K.R., Swinburne, I.A., Obholzer, N.D., Megason, S.G.
- ID
- ZDB-PUB-180227-17
- Date
- 2017
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 246: 451-465 (Journal)
- Registered Authors
- Megason, Sean, Mosaliganti, Kishore, Obholzer, Nikolaus, Swinburne, Ian
- Keywords
- inner ear, otic vesicle, regeneration, regulation, size control, symmetry
- MeSH Terms
-
- Animals
- Ear, Inner/anatomy & histology
- Ear, Inner/embryology
- Ear, Inner/growth & development*
- Feedback, Physiological
- Microscopy, Confocal
- Morphogenesis*
- Organogenesis/physiology*
- Time
- Zebrafish
- PubMed
- 28295855 Full text @ Dev. Dyn.
Citation
Green, A.A., Mosaliganti, K.R., Swinburne, I.A., Obholzer, N.D., Megason, S.G. (2017) Recovery of shape and size in a developing organ pair. Developmental Dynamics : an official publication of the American Association of Anatomists. 246:451-465.
Abstract
Background Paired organs in animals are largely bilaterally symmetric despite inherent noise in most biological processes. How is precise organ shape and size achieved during development despite this noise? Examining paired organ development is a challenge because it requires repeated quantification of two structures in parallel within living embryos. Here we combine bilateral quantification of morphology through time with asymmetric perturbations to study regulation of organ shape, size, and symmetry in developing organ pairs.
Results We present quantitative live imaging tools to measure the shape and size of the developing inner ears on both the left and right side simultaneously over time. By quantifying variation between the left and right inner ear (intrinsic noise) and between different individuals (extrinsic noise), we find that initial variability decreases over time in normal development to achieve symmetry. Early asymmetry is increased by environmental stress, but symmetry is still recovered over subsequent developmental time. Using multiple unilateral perturbations including Fgf signaling and ultraviolet light, we find that growth can be adjusted to compensate for a range of initial size and shape differences.
Conclusions We propose that symmetry in developmental systems does not emerge through precise deterministic bilateral development, but rather through feedback mechanisms that adjust morphogenesis rates to account for variation. Developmental Dynamics 246:451-465, 2016. © 2017 Wiley Periodicals, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping