PUBLICATION
TrkB receptor antagonism inhibits stab injury induced proliferative response in adult zebrafish (Danio rerio) brain
- Authors
- Anand, S.K., Mondal, A.C.
- ID
- ZDB-PUB-180223-62
- Date
- 2018
- Source
- Neuroscience letters 672: 28-33 (Journal)
- Registered Authors
- Keywords
- Adult zebrafish, BDNF, Brain regeneration, Stab injury, TrkB
- MeSH Terms
-
- Proliferating Cell Nuclear Antigen/metabolism
- Receptor, trkB/antagonists & inhibitors*
- Receptor, trkB/metabolism
- Brain/drug effects*
- Brain/metabolism
- Cell Proliferation/drug effects*
- Animals
- Benzamides/pharmacology*
- Wound Healing/drug effects*
- Zebrafish
- Azepines/pharmacology*
- PubMed
- 29471003 Full text @ Neurosci. Lett.
Citation
Anand, S.K., Mondal, A.C. (2018) TrkB receptor antagonism inhibits stab injury induced proliferative response in adult zebrafish (Danio rerio) brain. Neuroscience letters. 672:28-33.
Abstract
The Tropomyosin related kinase (TrkB) receptor, is known to promote neuronal maturation, differentiation, maintenance and survival through its cognate ligands Brain derived neurotrophic factor (BDNF) and neurotrophin 4 (NT4). BDNF, NT4 and TrkB are highly conserved proteins among vertebrates. Although the role of TrkB during brain development is well established, its role in adult neurogenesis and brain regeneration awaits thorough investigation. In this study, we used the zebrafish stab wound injury model to determine whether the injury induced regeneration response in the telencephalon region is governed by TrkB or not. We induced stab wound injury in the mid-dorsal region of telencephalon of ANA-12 (selective TrkB antagonist) treated and non-treated zebrafish brain and examined the proliferation activity in selected brain regions using immunohistochemistry. We found that proliferation activity was significantly low in ANA-12 injected injured fishes as compared to vehicle control injured fishes. Other major findings of the study include the temporal pattern of proliferation activity after an injury and activation of adult neural stem cells (aNSCs) situated distantly apart from the injury site in the adult zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping