PUBLICATION
Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein
- Authors
- Sztal, T.E., McKaige, E.A., Williams, C., Ruparelia, A.A., Bryson-Richardson, R.J.
- ID
- ZDB-PUB-180209-7
- Date
- 2018
- Source
- PLoS Genetics 14: e1007212 (Journal)
- Registered Authors
- Bryson-Richardson, Robert, Ruparelia, Avnika, Sztal, Tamar Esther
- Keywords
- none
- MeSH Terms
-
- Actins/genetics*
- Animals
- Animals, Genetically Modified
- Dosage Compensation, Genetic/physiology*
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Muscle, Skeletal/metabolism
- Muscle, Skeletal/pathology*
- Mutation*
- Myopathies, Nemaline/genetics*
- Myopathies, Nemaline/pathology
- Penetrance
- Phenotype
- Protein Isoforms/genetics
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 29420541 Full text @ PLoS Genet.
Citation
Sztal, T.E., McKaige, E.A., Williams, C., Ruparelia, A.A., Bryson-Richardson, R.J. (2018) Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein. PLoS Genetics. 14:e1007212.
Abstract
The lack of a mutant phenotype in homozygous mutant individuals' due to compensatory gene expression triggered upstream of protein function has been identified as genetic compensation. Whilst this intriguing process has been recognized in zebrafish, the presence of homozygous loss of function mutations in healthy human individuals suggests that compensation may not be restricted to this model. Loss of skeletal ∞-actin results in nemaline myopathy and we have previously shown that the pathological symptoms of the disease and reduction in muscle performance are recapitulated in a zebrafish antisense morpholino knockdown model. Here we reveal that a genetic actc1b mutant exhibits mild muscle defects and is unaffected by injection of the actc1b targeting morpholino. We further show that the milder phenotype results from a compensatory transcriptional upregulation of an actin paralogue providing a novel approach to be explored for the treatment of actin myopathy. Our findings provide further evidence that genetic compensation may influence the penetrance of disease-causing mutations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping