PUBLICATION
Functional characterization of tumor necrosis factor receptor-associated factor 3 of sea perch (Lateolabrax japonicas) in innate immune
- Authors
- Zhang, W., Jia, P., Liu, W., Li, Y., Yi, M., Jia, K.
- ID
- ZDB-PUB-180207-12
- Date
- 2018
- Source
- Fish & shellfish immunology 75: 1-7 (Journal)
- Registered Authors
- Kuntong, Jia, Liu, Wei, Yi, Meisheng
- Keywords
- Innate immune, Nervous necrosis virus, RIG-I-like receptor, Sea perch, Tumor necrosis factor receptor-associated factor 3
- MeSH Terms
-
- Animals
- Fish Diseases/immunology*
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Gene Expression Profiling
- Gene Expression Regulation
- Immunity, Innate/genetics*
- Nodaviridae/physiology
- Perciformes/genetics*
- Perciformes/immunology*
- RNA Virus Infections/immunology
- Sequence Analysis, DNA/veterinary
- Signal Transduction/genetics*
- TNF Receptor-Associated Factor 3/genetics*
- TNF Receptor-Associated Factor 3/metabolism
- PubMed
- 29407611 Full text @ Fish Shellfish Immunol.
Citation
Zhang, W., Jia, P., Liu, W., Li, Y., Yi, M., Jia, K. (2018) Functional characterization of tumor necrosis factor receptor-associated factor 3 of sea perch (Lateolabrax japonicas) in innate immune. Fish & shellfish immunology. 75:1-7.
Abstract
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a multifunctional regulator implicated in both bacterial defense and antiviral immunity. Here, a TRAF3 gene from the seawater fish sea perch, designated as LjTRAF3, was characterized. The full-length cDNA of LjTRAF3 was 2972 bp including a 5' untranslated region (UTR) of 243 bp, a 3'UTR of 941 bp and a putative open reading frame of 1608 bp encoding a putative protein of 536 amino acid. The deduced LjTRAF3 protein contained a RING finger, two zinc fingers, a coiled-coil, and a meprin and TRAF-C homology domain. Phylogenetic analysis showed that LjTRAF3 shared the closest genetic relationship with Larimichthys crocea TRAF3. Gene expression analyses suggested that LjTRAF3 mRNA was ubiquitously expressed in all the tissues tested, and was up-regulated post red spotted grouper nervous necrosis virus (RGNNV) infection in vivo and in vitro. Reporter gene assay showed that LjTRAF3 significantly activated zebrafish type I interferon (IFN) promoter in vitro. During RGNNV infection, ectopic expression of LjTRAF3 significantly reduced the RNA dependent RNA polymerase transcription of RGNNV, and enhanced the expression of RIG-I-like receptors (RLR), janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathway related genes and IFN stimulated genes (ISG), including ISG15, PKR, VIG and TRIM39. Taken together, our results suggested that LjTRAF3 might trigger the expression of various ISGs to counter RGNNV infection by regulating the RLR-induced IFN and JAK-STAT signaling pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping