PUBLICATION
Chilling causes perivitelline granule formation in activated zebrafish oocytes
- Authors
- Jalali, F., DiBenedetto, A.J., Karlsson, J.O.M.
- ID
- ZDB-PUB-180207-11
- Date
- 2018
- Source
- Cryobiology 81: 210-213 (Journal)
- Registered Authors
- DiBenedetto, Angela
- Keywords
- Chilling, Cryomicroscopy, Cryopreservation, Danio rerio, Gamete, Oocyte, Teleost, Zebrafish
- MeSH Terms
-
- Animals
- Cryopreservation/veterinary*
- Female
- Oocytes/pathology*
- Zebrafish*
- PubMed
- 29407225 Full text @ Cryobiology
Citation
Jalali, F., DiBenedetto, A.J., Karlsson, J.O.M. (2018) Chilling causes perivitelline granule formation in activated zebrafish oocytes. Cryobiology. 81:210-213.
Abstract
Chilling sensitivity in oocytes of the zebrafish represents a potential obstacle to their successful cryopreservation. Here, we report the first cryomicroscopic observations of the response of zebrafish oocytes to chilling conditions. In activated stage V oocytes that had been exposed to hypothermic temperatures, we observed a latent effect of chilling, manifesting as a granular precipitate that appeared in the perivitelline fluid upon return to 28.5 °C. The granules were visible in unstained oocytes under transmitted light microscopy, and the resulting perivitelline turbidity increased in a dose-dependent manner with decreasing chilling temperature (p < 0.001), as well as with increasing time of hypothermic exposure (p < 0.0001). The change in appearance of the perivitelline space in oocytes that had been chilled and rewarmed became statistically significant after a 7-min exposure to 10 °C and after only 30 s at 1 °C (p < 0.05). Thus, even moderate chilling exposures can lead to detectable changes in activated zebrafish oocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping