PUBLICATION
Hypoxic Treatment of Zebrafish Embryos and Larvae
- Authors
- Kamei, H., Duan, C.
- ID
- ZDB-PUB-180114-6
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1742: 195-203 (Chapter)
- Registered Authors
- Duan, Cunming
- Keywords
- Developmental timing, Dissolved oxygen, Embryo, Hypoxic water, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects
- Hypoxia*
- Larva/growth & development
- Models, Animal*
- Zebrafish/embryology
- Zebrafish/growth & development*
- PubMed
- 29330801 Full text @ Meth. Mol. Biol.
Citation
Kamei, H., Duan, C. (2018) Hypoxic Treatment of Zebrafish Embryos and Larvae. Methods in molecular biology (Clifton, N.J.). 1742:195-203.
Abstract
Zebrafish has emerged as an informative animal model to study the biological impact and molecular mechanisms of hypoxia. Here we describe a simple method to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and can be applied to other fish and amphibian species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping