PUBLICATION
Simultaneous quantification of T4, T3, rT3, 3,5-T2 and 3,3'-T2 in larval zebrafish (Danio rerio) as a model to study exposure to polychlorinated biphenyls
- Authors
- Chen, X., Walter, K.M., Miller, G.W., Lein, P.J., Puschner, B.
- ID
- ZDB-PUB-180110-8
- Date
- 2018
- Source
- Biomedical chromatography : BMC 32(6): e4185 (Journal)
- Registered Authors
- Miller, Galen
- Keywords
- PCBs, UPLC-MS/MS, derivatization, thyroid hormones, zebrafish
- MeSH Terms
-
- Animals
- Chromatography, High Pressure Liquid
- Disease Models, Animal
- Endocrine Disruptors/toxicity
- Larva/drug effects*
- Larva/metabolism
- Limit of Detection
- Linear Models
- Polychlorinated Biphenyls/toxicity*
- Reproducibility of Results
- Tandem Mass Spectrometry
- Thyroid Hormones/analysis*
- Zebrafish
- PubMed
- 29314156 Full text @ Biomed. Chromatogr.
Citation
Chen, X., Walter, K.M., Miller, G.W., Lein, P.J., Puschner, B. (2018) Simultaneous quantification of T4, T3, rT3, 3,5-T2 and 3,3'-T2 in larval zebrafish (Danio rerio) as a model to study exposure to polychlorinated biphenyls. Biomedical chromatography : BMC. 32(6):e4185.
Abstract
Environmental toxicants that interfere with thyroid hormone (TH) signaling can impact growth and development in animals and humans. Zebrafish represent a model to study chemically induced TH disruption, prompting the need for sensitive detection of THs. Simultaneous quantification of 3,3',5-triiodo-l-thyronine (T3), thyroxine (T4), 3,3',5'-triiodo-l-thyronine (rT3), 3,5-diiodo-l-thyronine (3,5-T2) and 3,3'-diiodo-l-thyronine (3,3'-T2) in zebrafish larvae was achieved by ultra-performance liquid chromatography-tandem mass spectrometry in positive ion mode. Solid-phase extraction with SampliQ cartridges and derivatization with 3 m hydrochloric acid in n-butanol reduced matrix effects. Derivatized compounds were separated on an Acquity UPLC BEH C18 column with mobile phases consisting of 0.1% acetic acid in deionized water and 0.1% acetic acid in methanol. The limits of detection ranged from 0.5 to 0.6 pg injected on column. The method was validated by evaluating recovery (77.1-117.2%), accuracy (87.3-123.9%) and precision (0.5-12.4%) using diluted homogenized zebrafish embryos spiked with all target compounds. This method was then applied to zebrafish larvae collected after 114 h of exposure to polychlorinated biphenyls (PCBs), including PCB 28, PCB 66 and PCB 95, or the technical mixture Aroclor 1254. Exposure to PCB 28 and PCB 95 increased the T4:T3 ratio and decreased the T3:rT3 ratio, demonstrating that this method can effectively detect PCB-induced alterations in THs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping