PUBLICATION
Cell-accurate optical mapping across the entire developing heart
- Authors
- Weber, M., Scherf, N., Meyer, A.M., Panáková, D., Kohl, P., Huisken, J.
- ID
- ZDB-PUB-171230-5
- Date
- 2017
- Source
- eLIFE 6: (Journal)
- Registered Authors
- Huisken, Jan, Meyer, Alexander, Panáková, Daniela
- Keywords
- Cardiac conduction, Cardiology, Light sheet microscopy, Optical mapping, Organogenesis, biophysics, developmental biology, image analysis, stem cells, structural biology, zebrafish
- MeSH Terms
-
- Animals
- Heart/embryology*
- Imaging, Three-Dimensional/methods*
- Intravital Microscopy/methods*
- Optical Imaging/methods*
- Organogenesis
- Zebrafish/embryology*
- PubMed
- 29286002 Full text @ Elife
Citation
Weber, M., Scherf, N., Meyer, A.M., Panáková, D., Kohl, P., Huisken, J. (2017) Cell-accurate optical mapping across the entire developing heart. eLIFE. 6.
Abstract
Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca2+-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping