PUBLICATION

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Authors
Ellett, F., Irimia, D.
ID
ZDB-PUB-171230-11
Date
2017
Source
Journal of visualized experiments : JoVE   (130): (Journal)
Registered Authors
Ellett, Felix
Keywords
none
MeSH Terms
  • Animals
  • Larva
  • Microinjections/methods*
  • Zebrafish/growth & development*
PubMed
29286475 Full text @ J. Vis. Exp.
Abstract
Zebrafish have emerged as a powerful model of various human diseases and a useful tool for an increasing range of experimental studies, spanning fundamental developmental biology through to large-scale genetic and chemical screens. However, many experiments, especially those related to infection and xenograft models, rely on microinjection and imaging of embryos and larvae, which are laborious techniques that require skill and expertise. To improve the precision and throughput of current microinjection techniques, we developed a series of microstructured devices to orient and stabilize zebrafish embryos at 2 days post fertilization (dpf) in ventral, dorsal, or lateral orientation prior to the procedure. To aid in the imaging of embryos, we also designed a simple device with channels that orient 4 zebrafish laterally in parallel against a glass cover slip. Together, the tools that we present here demonstrate the effectiveness of photolithographic approaches to generate useful devices for the optimization of zebrafish techniques.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes