PUBLICATION

Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1

Authors
Kimelman, D., Smith, N.L., Lai, J.K.H., Stainier, D.Y.
ID
ZDB-PUB-171229-4
Date
2017
Source
eLIFE   6: (Journal)
Registered Authors
Kimelman, David, Stainier, Didier
Keywords
developmental biology, stem cells, zebrafish
Datasets
GEO:GSE102606
MeSH Terms
  • Animals
  • Epidermis/embryology*
  • Fibronectins/metabolism
  • Gene Expression Regulation, Developmental*
  • Intracellular Signaling Peptides and Proteins/metabolism*
  • Morphogenesis*
  • Notochord/embryology*
  • Trans-Activators/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism*
PubMed
29283341 Full text @ Elife
Abstract
The vertebrate embryo undergoes a series of dramatic morphological changes as the body extends to form the complete anterior-posterior axis during the somite-forming stages. The molecular mechanisms regulating these complex processes are still largely unknown. We show that the Hippo pathway transcriptional coactivators Yap1 and Wwtr1 are specifically localized to the presumptive epidermis and notochord, and play a critical and unexpected role in posterior body extension by regulating Fibronectin assembly underneath the presumptive epidermis and surrounding the notochord. We further find that Yap1 and Wwtr1, also via Fibronectin, have an essential role in the epidermal morphogenesis necessary to form the initial dorsal and ventral fins, a process previously thought to involve bending of an epithelial sheet, but which we now show involves concerted active cell movement. Our results reveal how the Hippo pathway transcriptional program, localized to two specific tissues, acts to control essential morphological events in the vertebrate embryo.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping