PUBLICATION
Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry
- Authors
- Eryılmaz, O., Ateş, P.S., Ünal, İ., Üstündağ, Ü.V., Bay, S., Alturfan, A.A., Yiğitbaşı, T., Emekli-Alturfan, E., Akalın, M.
- ID
- ZDB-PUB-171229-3
- Date
- 2017
- Source
- Journal of biochemical and molecular toxicology 32(1): (Journal)
- Registered Authors
- Ateş, Perihan Seda, Emekli-Alturfan, Ebru, Unal, Ismail, Üstündağ, Ünsal Veli
- Keywords
- Wnt pathway, apoptosis, oxidant-antioxidant status, silver nanoparticles, zebrafish embryo
- MeSH Terms
-
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Cyclin D1/antagonists & inhibitors
- Cyclin D1/genetics
- Cyclin D1/metabolism
- Oxidative Stress/drug effects*
- Glycogen Synthase Kinase 3 beta/chemistry
- Glycogen Synthase Kinase 3 beta/genetics
- Glycogen Synthase Kinase 3 beta/metabolism
- Dose-Response Relationship, Drug
- Cell Proliferation/drug effects
- Animals
- Gene Expression Regulation, Developmental/drug effects
- Metal Nanoparticles/administration & dosage
- Metal Nanoparticles/toxicity*
- Enzyme Induction/drug effects
- Zebrafish
- Lipid Peroxidation/drug effects
- Silver/administration & dosage
- Silver/toxicity*
- Zebrafish Proteins/agonists
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Wnt Signaling Pathway/drug effects*
- Teratogens/toxicity
- Embryonic Development/drug effects
- Apoptosis/drug effects*
- Disinfectants/toxicity
- Water Pollutants, Chemical/administration & dosage
- Water Pollutants, Chemical/toxicity*
- PubMed
- 29283201 Full text @ J. Biochem. Mol. Toxicol.
- CTD
- 29283201
Citation
Eryılmaz, O., Ateş, P.S., Ünal, İ., Üstündağ, Ü.V., Bay, S., Alturfan, A.A., Yiğitbaşı, T., Emekli-Alturfan, E., Akalın, M. (2017) Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry. Journal of biochemical and molecular toxicology. 32(1).
Abstract
Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) are nanotechnology products with antimicrobial properties. However, exposure to nanoparticles in daily life is an important issue for public health, still being updated. Aim was to evaluate the effects of AgNPs on the development of zebrafish embryos focusing on Wnt pathway, proliferation, oxidant-antioxidant status, and apoptosis. The expressions of ccnd1 and gsk3β were determined by RT-PCR, whereas β-catenin and proliferative cell antigen (PCNA) expressions were determined immunohistochemically. Lipid peroxidation, superoxide dismutase, and glutathione-S-transferase activities were determined spectrophotometrically. Apoptosis was determined using acridine orange staining. Oxidant status, apoptosis, immunohistochemical PCNA, and β catenin staining increased, whereas ccnd1 and antioxidant enzyme activities decreased in AgNPs-exposed embryos in a dose-dependent manner. Our results indicate the interaction of possible mechanisms that may be responsible for the toxic effects of AgNPs in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping