PUBLICATION
Three-dimensional live multi-label light-sheet imaging with synchronous excitation-multiplexed structured illumination
- Authors
- Xu, D., Zhou, W., Peng, L.
- ID
- ZDB-PUB-171219-1
- Date
- 2017
- Source
- Optics express 25: 31159-31173 (Journal)
- Registered Authors
- Zhou, Weibin
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 29245793 Full text @ Opt. Express
Citation
Xu, D., Zhou, W., Peng, L. (2017) Three-dimensional live multi-label light-sheet imaging with synchronous excitation-multiplexed structured illumination. Optics express. 25:31159-31173.
Abstract
Multiplexed imaging is a powerful tool for studying complex interactions inside biological systems. Spectral imaging methods that capture multiple fluorescent markers synchronously without sacrificing the imaging speed or resolution are most suitable for live imaging. We describe spectral-encoded structured illumination (spectral-SIM) light-sheet microscopy, which enables parallel multi-excitation-channel imaging in 3D. Spectral-SIM encodes the excitation wavelength as the phase of the illumination pattern, and allows synchronous image capture over multiple excitation channels at the same speed and spatial resolution as mono-channel structured light-sheet imaging. The technique retains structured light-sheet microscopy's ability in removing out-of-focus and scattered emission background, and generates clear 3D multiplexed images in thick tissue. The capability of this technique was demonstrated by the imaging of live triple-labeled transgenic zebrafish to over 300 μm deep with 0.5μm-by-2μm (lateral-by-axial) resolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping