PUBLICATION
Chemosuppressive effect of plumbagin on human non-small lung cancer cell xenotransplanted zebrafish
- Authors
- Vinothkumar, R., Ceasar, S.A., Divyarupa, A.
- ID
- ZDB-PUB-171205-5
- Date
- 2017
- Source
- Indian journal of cancer 54: 253-256 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Antineoplastic Agents, Phytogenic/administration & dosage
- Antineoplastic Agents, Phytogenic/chemistry
- Carcinoma, Non-Small-Cell Lung/drug therapy*
- Carcinoma, Non-Small-Cell Lung/genetics
- Carcinoma, Non-Small-Cell Lung/pathology
- Cell Line, Tumor
- Cell Proliferation/drug effects*
- Gene Expression Regulation, Neoplastic/drug effects
- Humans
- Mice
- Naphthoquinones/administration & dosage*
- Naphthoquinones/chemistry
- Plumbaginaceae/chemistry
- Signal Transduction/drug effects
- Transplantation, Heterologous
- Tumor Suppressor Protein p53/genetics*
- Zebrafish/genetics
- PubMed
- 29199700 Full text @ Indian J Cancer
Citation
Vinothkumar, R., Ceasar, S.A., Divyarupa, A. (2017) Chemosuppressive effect of plumbagin on human non-small lung cancer cell xenotransplanted zebrafish. Indian journal of cancer. 54:253-256.
Abstract
Background Plumbagin (5-hydroxy-2-methyl-1,4-napthoquinone) derived from Plumbago species is a potential anti-tumour agent. Plumbagin has been tested for anti-cancer activity in vitro and in vivo using mice model.
Aim To study the tumour suppressing efficacy of plumbagin using zebrafish model.
Materials and methods Human Non-small lung cancer cell line were cultured in vitro and transplanted in to zebrafish. The development of tumour was confirmed by performing histology. The tumour was then allowed to progress in vivo and the fishes were administered with plumbagin orally for three continuous days. The tumour suppression capacity was monitored subsequently using transcriptosome analysis.
Statistical methods The pixel integrated density obtained was converted into relative gene expression using IBM SPSS.
Results The administration of plumbagin had an ability to suppress tumour and the size of the tumour were relatively lesser when compared with the control sample; it has also increased p53 gene expression.
Conclusion The study helps to conclude that plumbagin is an effective anti-tumour agent against human cancer cells based on the study in vivo in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping