PUBLICATION
Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtg1) mRNA expression
- Authors
- Chen, M., Zhang, J., Pang, S., Wang, C., Wang, L., Sun, Y., Song, M., Liang, Y.
- ID
- ZDB-PUB-171205-3
- Date
- 2017
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 204: 45-50 (Journal)
- Registered Authors
- Chen, Minjie, Liang, Yong, Pang, Shaochen, Sun, Yonghua
- Keywords
- Endocrine disrupting chemicals, Estrogenic effect, Vitellogenin 1, Zebrafish embryo
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Endocrine Disruptors/toxicity*
- Estradiol/pharmacology
- Gene Expression Regulation, Developmental/drug effects*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Vitellogenins/genetics
- Vitellogenins/metabolism*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29199129 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
- CTD
- 29199129
Citation
Chen, M., Zhang, J., Pang, S., Wang, C., Wang, L., Sun, Y., Song, M., Liang, Y. (2017) Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtg1) mRNA expression. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 204:45-50.
Abstract
By measuring the vitellogenin 1 (vtg1) expression through quantitative PCR and in situ hybridization, we used the zebrafish embryo as an in vivo model to access the estrogenic or anti-estrogenic effects of several endocrine disrupting chemicals (EDCs), such as natural estrogen 17β-estradiol (E2), estriol (E3), synthetic hormones including diethylstilbestrol (DES), 4-octyl phenol (OP), bisphenol A (BPA), tamoxifen (TMX) and 3-(2,3-dibromopropyl) isocyanurate (TBC). According to our data, the estrogenic effect of the tested chemicals was ranked as: DES>E2>E3>OP>BPA, which is consistent with various in vivo and in vitro models. Therefore, the measurement of vtg1 gene expression in zebrafish embryos would be a valuable method for screening EDCs including both environmental estrogens and anti-estrogens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping