PUBLICATION
Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation
- Authors
- King, J., Foster, J., Davison, J.M., Rawls, J.F., Breton, G.
- ID
- ZDB-PUB-171128-3
- Date
- 2017
- Source
- Zebrafish 15(2): 202-205 (Journal)
- Registered Authors
- Breton, Ghislain, Davison, James M., Rawls, John F.
- Keywords
- ORFeome, eY1H, yeast one hybrid
- MeSH Terms
-
- Animals
- Cloning, Molecular
- Computational Biology
- Gene Expression Regulation
- Gene Library
- Gene Regulatory Networks*
- Open Reading Frames*
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 29173090 Full text @ Zebrafish
Citation
King, J., Foster, J., Davison, J.M., Rawls, J.F., Breton, G. (2017) Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation. Zebrafish. 15(2):202-205.
Abstract
The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid interaction techniques to provide unprecedented discovery capabilities for protein-DNA interactions. Unfortunately its use for zebrafish research is currently hampered by the lack of an open reading frame (ORF) clone collection. As a first step toward a complete collection, we describe a small library of transcriptional regulatory proteins comprising 142 ORFs and its potential applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping