PUBLICATION
Polyphenols from Euphorbia pekinensis Inhibit AGEs Formation In Vitro and Vessel Dilation in Larval Zebrafish In Vivo
- Authors
- Lee, I.S., Jung, S.H., Kim, J.S.
- ID
- ZDB-PUB-171123-8
- Date
- 2017
- Source
- Planta Medica 84(3): 176-181 (Journal)
- Registered Authors
- Jung, Seung-Hyun
- Keywords
- none
- MeSH Terms
-
- Animals
- Chemical Fractionation
- Ellagic Acid/analogs & derivatives
- Euphorbia/chemistry*
- Glycation End Products, Advanced/drug effects*
- Hypoglycemic Agents/chemistry
- Hypoglycemic Agents/isolation & purification
- Hypoglycemic Agents/pharmacology*
- Larva/drug effects
- Molecular Structure
- Polyphenols/chemistry
- Polyphenols/isolation & purification
- Polyphenols/pharmacology*
- Retinal Vessels/drug effects
- Vasodilation/drug effects
- Zebrafish
- PubMed
- 29165729 Full text @ Planta Med.
Citation
Lee, I.S., Jung, S.H., Kim, J.S. (2017) Polyphenols from Euphorbia pekinensis Inhibit AGEs Formation In Vitro and Vessel Dilation in Larval Zebrafish In Vivo. Planta Medica. 84(3):176-181.
Abstract
To identify active compounds in the roots of Euphorbia pekinensis for treatment of diabetic complications, an active column fraction from a 70% EtOH extract of E. pekinensis root was purified by preparative reversed-phase high-performance liquid chromatography, leading to the isolation of a new ellagic acid derivative, 3,3'-di-O-methylellagic acid 4-O-(6"-O-galloyl)-β-D-galactopyranoside (1: ), along with three known compounds, geraniin (2: ), 3,3'-di-O-methylellagic acid 4-O-β-D-xylopyranoside (3: ), and ellagic acid 3,3'-dimethyl ether (4: ). The structure of the new compound was established by extensive spectroscopic studies and chemical evidence. The inhibitory effects of isolated compounds 1: -4: on advanced glycation end-products (AGEs) formation were examined. All compounds exhibited considerable inhibition of AGEs formation and IC50 values of 0.41 - 12.33 µM, compared with those of the positive controls aminoguanidine (IC50 = 1122.34 µM) and quercetin (IC50 = 27.80 µM). In addition, the effects of 2: and 4: on the dilation of hyaloid-retinal vessels induced by high glucose (HG) in larval zebrafish were investigated; both compounds significantly reduced the HG-induced dilation of hyaloid-retinal vessels relative to the HG-treated control group.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping