PUBLICATION
Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1
- Authors
- Lewis, T.R., Zareba, M., Link, B.A., Besharse, J.C.
- ID
- ZDB-PUB-171117-13
- Date
- 2017
- Source
- Molecular biology of the cell 29(2): 180-190 (Journal)
- Registered Authors
- Besharse, Joseph C., Lewis, Tylor, Link, Brian
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cytoplasmic Dyneins/physiology*
- Dark Adaptation
- Microtubules/physiology*
- Microtubules/ultrastructure
- Nocodazole/pharmacology
- Pigment Epithelium of Eye
- Retinal Cone Photoreceptor Cells/physiology*
- Retinal Cone Photoreceptor Cells/ultrastructure
- Tubulin Modulators/pharmacology
- Zebrafish/anatomy & histology*
- PubMed
- 29142075 Full text @ Mol. Biol. Cell
Citation
Lewis, T.R., Zareba, M., Link, B.A., Besharse, J.C. (2017) Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1. Molecular biology of the cell. 29(2):180-190.
Abstract
Teleosts and amphibians exhibit retinomotor movements, morphological changes in photoreceptors regulated by light and circadian rhythms. Cone myoid elongation occurs during dark adaptation leading to the positioning of the cone outer segment closer to the retinal pigment epithelium. While it has been shown that microtubules are essential for cone myoid elongation, the underlying mechanism has not been established. In this work, we generated a transgenic line of zebrafish expressing a photoconvertible form of α-tubulin (tdEOS-tubulin) specifically in cone photoreceptors. Using super-resolution structured illumination microscopy (SIM) in conjunction with both pharmacological and genetic manipulation, we show that cytoplasmic dynein-1, which localizes to the junction between the ellipsoid and myoid, functions to shuttle microtubules from the ellipsoid into the myoid during the course of myoid elongation. We propose a novel model by which stationary complexes of cytoplasmic dynein-1 are responsible for the shuttling of microtubules between the ellipsoid and myoid is the underlying force for the morphological change of myoid elongation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping