PUBLICATION

Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors.

Authors
Brockerhoff, S.E.
ID
ZDB-PUB-171114-4
Date
2017
Source
Advances in experimental medicine and biology   1016: 91-100 (Chapter)
Registered Authors
Brockerhoff, Susan
Keywords
Calcium, Genome editing, Mitochondria, Photoreceptors, Zebrafish
MeSH Terms
  • Animals
  • CRISPR-Cas Systems/genetics*
  • Calcium Signaling/genetics*
  • Gene Editing*
  • Genome
  • Homeostasis/genetics
  • Humans
  • Retinal Cone Photoreceptor Cells/metabolism*
  • Retinal Cone Photoreceptor Cells/pathology
  • Zebrafish/genetics
PubMed
29130155 Full text @ Adv. Exp. Med. Biol.
Citation
Brockerhoff, S.E. (2017) Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors.. Advances in experimental medicine and biology. 1016:91-100.
Abstract
Photoreceptors are specialized sensory neurons with unique biological features. Phototransduction is well understood due in part to the exclusive expression and function of the molecular components of this cascade. Many other processes are less well understood, but also extremely important for understanding photoreceptor function and for treating disease. One example is the role of Ca2+ in the cell body and overall compartmentalization and regulation of Ca2+ within the cell. The recent development of CRISPR/Cas9 genome editing techniques has made it possible to rapidly and cheaply alter specific genes. This will help to define the biological function of elusive processes that have been more challenging to study. CRISPR/Cas9 has been optimized in many systems including zebrafish, which already has some distinct advantages for studying photoreceptor biology and function. These new genome editing technologies and the continued use of the zebrafish model system will help advance our understanding of important understudied aspects of photoreceptor biology.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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