Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors.
- Brockerhoff, S.E.
- Advances in experimental medicine and biology 1016: 91-100 (Chapter)
- Registered Authors
- Brockerhoff, Susan
- Calcium, Genome editing, Mitochondria, Photoreceptors, Zebrafish
- MeSH Terms
- CRISPR-Cas Systems/genetics*
- Calcium Signaling/genetics*
- Gene Editing*
- Retinal Cone Photoreceptor Cells/metabolism*
- Retinal Cone Photoreceptor Cells/pathology
- 29130155 Full text @ Adv. Exp. Med. Biol.
Brockerhoff, S.E. (2017) Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors.. Advances in experimental medicine and biology. 1016:91-100.
Photoreceptors are specialized sensory neurons with unique biological features. Phototransduction is well understood due in part to the exclusive expression and function of the molecular components of this cascade. Many other processes are less well understood, but also extremely important for understanding photoreceptor function and for treating disease. One example is the role of Ca2+ in the cell body and overall compartmentalization and regulation of Ca2+ within the cell. The recent development of CRISPR/Cas9 genome editing techniques has made it possible to rapidly and cheaply alter specific genes. This will help to define the biological function of elusive processes that have been more challenging to study. CRISPR/Cas9 has been optimized in many systems including zebrafish, which already has some distinct advantages for studying photoreceptor biology and function. These new genome editing technologies and the continued use of the zebrafish model system will help advance our understanding of important understudied aspects of photoreceptor biology.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes