PUBLICATION
Functional characterization of apoptosis-associated speck-like protein (ASC) of the goldfish (Carassius auratus L.)
- Authors
- Xie, J., Belosevic, M.
- ID
- ZDB-PUB-171110-7
- Date
- 2016
- Source
- Developmental and comparative immunology 65: 201-210 (Journal)
- Registered Authors
- Keywords
- ASC, Fish, Inflammasome, Macrophage, Macrophages, Teleost
- MeSH Terms
-
- Protein Binding
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- Inflammasomes/metabolism*
- Caspase 1/metabolism
- Nigericin/pharmacology
- Goldfish/immunology*
- Protein Multimerization
- NLR Proteins/metabolism
- Animals
- Macrophages/physiology*
- Spleen/physiology*
- Up-Regulation
- Cells, Cultured
- Zebrafish Proteins/genetics
- Signal Transduction
- PubMed
- 27451255 Full text @ Dev. Comp. Immunol.
Citation
Xie, J., Belosevic, M. (2016) Functional characterization of apoptosis-associated speck-like protein (ASC) of the goldfish (Carassius auratus L.). Developmental and comparative immunology. 65:201-210.
Abstract
Quantitative expression analysis of goldfish ASC indicated the highest and lowest mRNA levels in spleen and muscle, respectively. The ASC was differentially expressed in normal goldfish tissues and different immune cell populations. The highest ASC mRNA levels were observed in the spleen and macrophages. We generated a recombinant form of the molecule (rgfASC) and an anti-ASC IgG antibody, and report that treatment of goldfish macrophages with nigericin, an inducer of inflammasome pathway, up-regulated the expression of ASC at both mRNA and protein levels. rgfASC aggregated to form multimers in cross-linking assays, and formed speck-like structures visualized by confocal microscopy. Co-immunoprecipitation assays showed that rgfASC interacted with caspase-1 and receptor-interacting serine/threonine kinase 2 (RIP2). The dual luciferase reporter assay showed that ASC over-expression did not cause the activation of NF-?B directly, but down-regulated RIP2 ability to activate NF-?B. Goldfish ASC was found to interact with both Nod-like receptor and inflammasome signaling pathway molecules, suggesting multifunctional roles for ASC in regulation of different NLR signaling pathways and eventual proinflammatory cytokine production by activated macrophages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping