PUBLICATION
3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
- Authors
- Dueñas, M.E., Essner, J.J., Lee, Y.J.
- ID
- ZDB-PUB-171104-5
- Date
- 2017
- Source
- Scientific Reports 7: 14946 (Journal)
- Registered Authors
- Essner, Jeffrey
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/chemistry*
- Embryo, Nonmammalian/cytology
- Imaging, Three-Dimensional/methods
- Phospholipids/analysis*
- Single-Cell Analysis/methods*
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*
- Zebrafish/embryology*
- PubMed
- 29097697 Full text @ Sci. Rep.
Citation
Dueñas, M.E., Essner, J.J., Lee, Y.J. (2017) 3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish. Scientific Reports. 7:14946.
Abstract
The zebrafish (Danio rerio) has been widely used as a model vertebrate system to study lipid metabolism, the roles of lipids in diseases, and lipid dynamics in embryonic development. Here, we applied high-spatial resolution matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) to map and visualize the three-dimensional spatial distribution of phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamines (PE), and phosphatidylinositol (PI), in newly fertilized individual zebrafish embryos. This is the first time MALDI-MSI has been applied for three dimensional chemical imaging of a single cell. PC molecular species are present inside the yolk in addition to the blastodisc, while PE and PI species are mostly absent in the yolk. Two-dimensional MSI was also studied for embryos at different cell stages (1-, 2-, 4-, 8-, and 16-cell stage) to investigate the localization changes of some lipids at various cell developmental stages. Four different normalization approaches were compared to find reliable relative quantification in 2D- and 3D- MALDI MSI data sets.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping