|ZFIN ID: ZDB-PUB-171031-5|
High-Throughput Automated Chemical Screens in Zebrafish
Saydmohammed, M., Tsang, M.
|Source:||Methods in molecular biology (Clifton, N.J.) 1683: 383-393 (Chapter)|
|Registered Authors:||Tsang, Michael|
|Keywords:||Chemical genetics, Drug discovery, FGF hyper-activators, Small molecule screens, Transgenic reporter|
|PubMed:||29082504 Full text @ Meth. Mol. Biol.|
Saydmohammed, M., Tsang, M. (2018) High-Throughput Automated Chemical Screens in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1683:383-393.
ABSTRACTZebrafish are increasingly used to perform phenotypic screens to identify agents that can alter physiology in a whole organismal context. Here, we describe an automated high-content chemical screen using transgenic zebrafish embryos to identify small molecules that modulate Fibroblast Growth Factor Signaling. High content multi-well screening was further refined with a particular emphasis on automated imaging and quantification that increases sensitivity and throughput of whole organism chemical screens.
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