PUBLICATION
In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish
- Authors
- Hsieh, J.Y., Papazian, D.M.
- ID
- ZDB-PUB-171024-5
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1684: 237-252 (Chapter)
- Registered Authors
- Papazian, Diane M.
- Keywords
- Cerebellum, Complex spike, Loose patch, Patch clamp, Purkinje cell, Spinocerebellar ataxia, Tonic firing, Zebrafish
- MeSH Terms
-
- Action Potentials
- Animals
- Animals, Genetically Modified
- Patch-Clamp Techniques
- Purkinje Cells/physiology*
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- PubMed
- 29058196 Full text @ Meth. Mol. Biol.
Citation
Hsieh, J.Y., Papazian, D.M. (2018) In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish. Methods in molecular biology (Clifton, N.J.). 1684:237-252.
Abstract
Zebrafish is a lower vertebrate model organism that facilitates integrative analysis of the in vivo effects of potassium and other ion channel mutations at the molecular, cellular, developmental, circuit, systems, and behavioral levels of analysis. Here, we describe a method for extracellular, loose patch electrophysiological recording of electrical activity in cerebellar Purkinje cells in living, awake zebrafish, with the goal of investigating pathological mechanisms underlying channelopathies or other diseases that disrupt cerebellar function. Purkinje cell excitability and a functional cerebellar circuit develop rapidly in zebrafish and show strong conservation with the mammalian cerebellum.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping